The fully authenticated human NCI-60 cancer cell lines (NCI, Bethesda, MD, USA) used in this study were cultured in RPMI media (Corning, 10–040-CV) supplemented with 10% FBS and 1% penicillin and streptomycin. Cancer cells were seeded a day before stimulation. On the day of stimulation, the cells were washed once with warm and sterile DPBS, followed by treatment with different cytokines for 48 h. The concentration of the cytokines used were 25 ng/mL of IL-6 (Peprotech, 200-06), IL-8 (Peprotech, 200-08M), IL-18 (R&D, B001-5), IL-15 (Peprotech, 200-15), IL-1α (Peprotech, 200-01A), IL-1β (Peprotech, 200-01B), IL-2 (Peprotech, 200-02), TNF-α (Peprotech, 300-01A), IFN-γ (Peprotech, 300-02). For the inhibition of nitric oxide (NO), cancer cells were co-treated with 1 mM of L-NAME hydrochloride (TOCRIS, 0665) or 100 μM of 1400W dihydrochloride (Enzo Life Sciences, ALX-270–073-M005) along with the cytokines. For inhibition of the JAK signaling pathway, the cancer cells were co-treated with 5 μM of baricitinib (Achemblock, K12360) all through the experiment.
L name hydrochloride
Manufactured by Bio-Techne
L-NAME hydrochloride is a synthetic compound that functions as a nitric oxide synthase (NOS) inhibitor. It is commonly used in research applications to study the physiological roles of nitric oxide in various biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Interleukin 6 (il 6), by Thermo Fisher Scientific (2 mentions)
Interleukin 2 (il 2), by Thermo Fisher Scientific (2 mentions)
Il 1α, by Thermo Fisher Scientific (2 mentions)
Tnf α, by Thermo Fisher Scientific (2 mentions)
Ifn γ, by Thermo Fisher Scientific (2 mentions)
1400w dihydrochloride, by Enzo Life Sciences (2 mentions)
Il 1β, by Thermo Fisher Scientific (1 mentions)
Il 15, by Thermo Fisher Scientific (1 mentions)
Rpmi media, by Corning (1 mentions)
Il 18, by R&D Systems (1 mentions)
Ifn λ, by Thermo Fisher Scientific (1 mentions)
Il 18, by BioLegend (1 mentions)
Il 1β, by R&D Systems (1 mentions)
Il 15, by R&D Systems (1 mentions)
2 protocols using l name hydrochloride
1
Cytokine-Induced Cancer Cell Responses
2
Cytokine-Stimulated Macrophage Activation
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BMDMs were stimulated with the following cytokines where indicated unless otherwise noted: 20 ng/mL of IL-6 (Peprotech, 212–16), 10 ng/mL of IL-18 (BioLegend, 767004), 20 ng/mL of IL-15 (R&D, 447-ML), 20 ng/mL of IL-1α (Peprotech, 200–01A), 20 ng/mL of IL-1β (R&D, 201-LB-025), 20 ng/mL of IL-2 (Peprotech, 212–12), 25 ng/mL of TNF-α (Peprotech, 315–01A), 50 ng/mL of IFN-γ (Peprotech, 315–05), 50 ng/mL of IFN-α (PBL Assay, 12106–1), 50 ng/mL of IFN-β (PBL Assay, 12400–1), or 50 ng/mL of IFN-λ (Peprotech, 250–33). For human cells, 50 ng/mL of TNF-α (Peprotech, AF-300–01A) and 100 ng/mL of IFN-γ (Peprotech, 300–02) was used for the indicated time. For the inhibition of NO, cells were co-treated with 1 mM of L-NAME hydrochloride (TOCRIS, 0665) or 100 μM of 1400W dihydrochloride (Enzo Life Sciences, ALX-270–073-M005). SIN-1 chloride (TOCRIS, 0750) at the indicated concentrations was used as the NO donor.
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