For histological staining, sections were deparaffinized, dehydrated and subjected to Verhoeff–Van Gieson staining using standard kits according to manufacturer’s protocols. Images were generated using an Axio slide scanner (Carl Zeiss Microscopy, White Plains, NY, USA) at 20x resolution. Four random rectangle images at the medial layer were taken per tissue to grade elastin fragmentation, defined as focal fragmentation of elastin. The criteria for the histologic grading were modified from Schlatmann and Becker [13 (link)] (see Supplementary Material ).
Axio slide scanner
Manufactured by Zeiss
Sourced in United States, Germany
The Axio slide scanner is a high-performance imaging system designed for digital pathology applications. It is capable of scanning glass slides and converting them into high-resolution digital images. The Axio slide scanner provides fast and reliable digitization of samples with optimal image quality.
Automatically generated - may contain errors
4 protocols using axio slide scanner
1
Verhoeff-Van Gieson Staining for Elastin
2
SARS-CoV-2 Immunohistochemistry in Lung Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5μm sections of formalin-fixed, paraffin-embedded lung were mounted on charged glass slides, baked for one hour at 60°C, and passed through Xylene, graded ethanol, and double distilled water to remove paraffin and rehydrate tissue sections. A microwave was used for heat induced epitope retrieval. Slides were heated in a high pH solution (Vector Labs H-3301), rinsed in hot water and transferred to a heated low pH solution (Vector Labs H-3300) where they were allowed to cool to room temperature. Sections were washed in a solution of phosphate-buffered saline and fish gelatin (PBS-FSG) and transferred to a humidified chamber, for staining at room temperature. Tissues were blocked with 10% normal goat serum (NGS) for 40 minutes, followed by a 60-minute incubation with a guinea pig anti-SARS antibody (BEI NR-10361) diluted 1:1000 in NGS. Slides were washed and transferred to the humidified chamber for a 40-minute incubation with a goat anti-guinea pig secondary antibody (Invitrogen A11073) tagged with Alexa Fluor 488 and diluted 1:1000 in NGS. Following washes, DAPI (4’,6-diamidino-2-phenylindole) was used to label the nuclei of each section. Slides were mounted using a homemade anti-quenching mounting media containing Mowiol (Calbiochem#475904) and DABCO (Sigma #D2522) and imaged at 20X with a Zeiss Axio Slide Scanner.
3
Fluorescent Imaging of Tumor Microenvironment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An activation buffer (0.1 M sodium acetate-acetic acid) or phosphate-buffered saline (PBS) was added to human plasma with a certain concentration of ONM-100 and mixed with 20X PBS in a 96-well plate. The fluorescence was measured using a plate reader (TECAN Infinite M200 PRO, Männedorf, Switzerland).
The tumor was collected with adjacent stromal tissue during surgery and immediately frozen in optimal cutting temperature compound (OCT). It was cut into 8 μm frozen sections and sprayed with a fluorescent pH sensor (ICG was substituted with a tetramethylrhodamine (TMR) dye) and incubated for 3 min. After 10 min of fixation with formalin, the slides were washed three times with 0.9% NaCL + 0.5% Tween 20. The sections were DAPI stained and scanned for fluorescence (Zeiss Axio slide scanner, Oberkochen, Germany). Adjacent slides were H/E stained for histopathological correlation purposes.
The tumor was collected with adjacent stromal tissue during surgery and immediately frozen in optimal cutting temperature compound (OCT). It was cut into 8 μm frozen sections and sprayed with a fluorescent pH sensor (ICG was substituted with a tetramethylrhodamine (TMR) dye) and incubated for 3 min. After 10 min of fixation with formalin, the slides were washed three times with 0.9% NaCL + 0.5% Tween 20. The sections were DAPI stained and scanned for fluorescence (Zeiss Axio slide scanner, Oberkochen, Germany). Adjacent slides were H/E stained for histopathological correlation purposes.
4
Multicolor Immunohistochemistry of Lung Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five μm sections of Formalin-fixed, paraffin-embedded lung were mounted on charged glass slides, baked overnight at 56 °C, and passed through Xylene, graded ethanol, and double distilled water to remove paraffin and rehydrate tissue sections. A microwave was used for heat-induced epitope retrieval, which was performed sequentially with high and low pH solutions from Vector Labs (H-3301 and H-3300). CD16+ and MPO+ cells were detected using MACH3 AP kits (Biocare Medical M3M532 or M3R533) and permanent red substrate (Dako K0640). MPO+ cells CD3+ cells CD11b+ cells CD68+/CD163+ cells, and CD206+ cells were detected using antibodies described in Supplementary Table 10 . Slides were incubated with a blocking buffer comprised of either 1% normal donkey serum or 10% normal goat serum for 40 min. All primary antibodies were incubated for 60 min and all secondary antibodies for 40 min at room temperature, with thorough washing in between. DAPI (4′,6-diamidino-2-phenylindole) was used to label the nuclei of each section. Slides were mounted using a homemade anti-quenching mounting media containing Mowiol (Calbiochem# 475904) and DABCO (Sigma# D2522) and imaged with a Zeiss Axio Slide Scanner.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!