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2 protocols using anti ve cadheline

1

Monocrotaline-Induced Pulmonary Fibrosis Model

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Monocrotaline (MCT) was purchased from Sigma-Aldrich Chemical Co., (St. Louis, MO). MCT was dissolved in 1 N HCl and at last adjusted pH to 7.4 by 1 N NaOH on each use. MCT pyrrole (MCTp) was purchased from Santa Cruz (Santa Cruz, CA) and dissolved in dimethylformamide. The stock solution of MCTp was kept at −80°C until use. Recombinant human TGF-β2 was purchased from Fujifilm Wako Pure Chemical (Osaka, Japan). Stealth RNAi of TRPM7 (M7 si1: HSS123307, M7 si2: HSS182662, M7 si3: HSS182663) and negative control siRNA were obtained from Invitrogen (Carlsbad, CA). FTY-720 was purchased from Cayman Chemical (Ann Arbor, MI) and dissolved in dimethyl sulfoxide. Antibodies against phospho-Smad2 (#3108, Cell Signaling Technology, Danvers, MA), anti-N-cadheline (#ab98952 Abcam, Cambridge, UK), anti-VE-cadheline (#ab33168 Abcam, Cambridge, UK), STAT3 (#9139, Cell Signaling Technology), phospho-STAT3 (#9138, Cell Signaling Technology), α-SMA (#ab32575, Abcam, Cambridge, UK), CD31 (#77699, Cell Signaling Technology), vWF (#ab6994, Abcam), TRPM7 (#ab85016, Abcam), Phospho-Akt (Ser473) (#4060, Cell Signaling Technology), Connexin 43 (#ab11370, Abcam) and β-actin (#ab6276, Abcam) were used for the immunoblotting and immunostaining experiments.
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2

Monocrotaline-Induced Pulmonary Fibrosis Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monocrotaline (MCT) was purchased from Sigma-Aldrich Chemical Co., (St. Louis, MO). MCT was dissolved in 1 N HCl and at last adjusted pH to 7.4 by 1 N NaOH on each use. MCT pyrrole (MCTp) was purchased from Santa Cruz (Santa Cruz, CA) and dissolved in dimethylformamide. The stock solution of MCTp was kept at −80°C until use. Recombinant human TGF-β2 was purchased from Fujifilm Wako Pure Chemical (Osaka, Japan). Stealth RNAi of TRPM7 (M7 si1: HSS123307, M7 si2: HSS182662, M7 si3: HSS182663) and negative control siRNA were obtained from Invitrogen (Carlsbad, CA). FTY-720 was purchased from Cayman Chemical (Ann Arbor, MI) and dissolved in dimethyl sulfoxide. Antibodies against phospho-Smad2 (#3108, Cell Signaling Technology, Danvers, MA), anti-N-cadheline (#ab98952 Abcam, Cambridge, UK), anti-VE-cadheline (#ab33168 Abcam, Cambridge, UK), STAT3 (#9139, Cell Signaling Technology), phospho-STAT3 (#9138, Cell Signaling Technology), α-SMA (#ab32575, Abcam, Cambridge, UK), CD31 (#77699, Cell Signaling Technology), vWF (#ab6994, Abcam), TRPM7 (#ab85016, Abcam), Phospho-Akt (Ser473) (#4060, Cell Signaling Technology), Connexin 43 (#ab11370, Abcam) and β-actin (#ab6276, Abcam) were used for the immunoblotting and immunostaining experiments.
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