IHC was performed using a Vectastain Elite kit (AK-5001, Vector Laboratories, Burlingame, CA) as described previously34 (link),42 (link),43 (link). Primary antibodies included the human-specific Ki67 (1:50) (Thermo Fisher, Waltham, MA), and rabbit anti-EZH2 (1:50) (Thermo Fisher, Waltham, MA) and H3K27me2 (1:100) (ABCAM INC, Cambridge, CA), and H3K27me3(1:50) (Thermo Fisher, Waltham, MA).
Anti ezh2
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-EZH2 is a lab equipment product designed for research purposes. It is a specific antibody targeting the EZH2 protein, which is involved in epigenetic regulation. The core function of this product is to facilitate the detection and analysis of EZH2 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Vectastain elite kit, by Vector Laboratories (1 mentions)
H3k27me3, by Thermo Fisher Scientific (1 mentions)
H3k27me2, by Abcam (1 mentions)
Anti eif4e, by Cell Signaling Technology (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Rabbit anti β tubulin antibody, by Cell Signaling Technology (1 mentions)
Anti p21, by Cell Signaling Technology (1 mentions)
2 protocols using anti ezh2
1
Immunohistochemistry of Epigenetic Markers
2
Evaluating Ribavirin's Effects on Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells treated with ribavirin or control were lysed and Western blots were performed using the following rabbit and mouse antibodies: anti-phospho (S209) eIF4E (1/1000, #9742, Cell Signaling, Beverly, MA, USA), anti-eIF4E (1/1000, #9741, Cell Signaling), anti-EZH2 (1/500, #MA5-18108, ThermoFisher Scientific, Waltham, MA USA), anti-phospho (T202/Y204) ERK (1/1000, #4370, Cell Signaling), anti-ERK (1/1000, #9102, Cell Signaling), and anti-p21 (1/1000, #2947, Cell Signaling). Data were normalized to their respective loading controls using a rabbit anti-β-tubulin antibody (1/1000, #2146, Cell Signaling). Gel quantification was performed using ImageJ software.
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