Human umbilical vein endothelial cells (HUVECs) were purchased from the Cell Bank of Shanghai Institute of Cell Biology, Chinese Academy of Sciences. Cells were cultured in Dulbecco's modified Eagle medium (C12430500BT, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco), 100-U/mL penicillin, and 100-mg/mL streptomycin (Gibco) in an incubator at 37°C with 5% CO2. HUVECs were treated with indicated concentrations of lipopolysaccharide (LPS) (L4391; Sigma-Aldrich Co. Ltd, MO, USA) for different times. To examine the role of PI3Kγ in LPS-treated endothelial cells, 2-μmol/L CZC24832 (S7018; Selleck Chemicals) was added to culture medium 2 h before the administration of LPS.
C12430500bt
Manufactured by Thermo Fisher Scientific
Sourced in United States
The C12430500BT is a piece of laboratory equipment manufactured by Thermo Fisher Scientific. It is designed to perform a core function within the laboratory setting. Due to the technical nature of this product, a detailed and unbiased description cannot be provided without the risk of extrapolation or interpretation. Therefore, the description for this product is not available.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Huvecs, by Merck Group (1 mentions)
Czc24832, by Selleck Chemicals (1 mentions)
C11995500bt, by Thermo Fisher Scientific (1 mentions)
2 protocols using c12430500bt
1
HUVEC Cultivation and LPS Treatment
2
Cell culture conditions for neuroblastoma studies
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All of the cell lines used in this study were purchased from the Kunming Cell Bank, Chinese Academy of Sciences (these cell lines were originally obtained from the ATCC). HEK293 cells were cultured in high-glucose Dulbecco's Modified Eagle's medium (DMEM; C11995500BT, Gibco) containing 10% fetal bovine serum (FBS; 10091148, Gibco), penicillin and streptomycin (100 U/ml)) (10378016, Gibco). SK-N-SH and SH-SY5Y cells were cultured in high-glucose DMEM (C12430500BT, Gibco) supplemented with 10% FBS, 10 mM sodium pyruvate solution (11360070, Gibco), 1× Minimum Essential Medium non-essential amino acid solution (11140050, Gibco), penicillin and streptomycin (100 U/ml). The antibiotics were withdrawn 48 h before performing assays and all cells were cultured at 37 °C with 5% CO2 and 95% air. No mycoplasma contamination was found for the cell lines used in this study.
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