Thrombin antithrombin complexes tatc

Manufactured by Siemens

Sourced in Germany

Thrombin-antithrombin complexes (TATc) are proteins formed when thrombin, a key enzyme in the blood coagulation process, binds to antithrombin, a natural anticoagulant. TATc are commonly used in laboratory settings to measure the activation of the coagulation system.

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Lab products found in correlation

Bradford based protein assay, by Bio-Rad (1 mentions) D dimer, by Roche (1 mentions) Cba multiplex assay, by BD (1 mentions) Cytometric bead array multiplex assay, by BD (1 mentions) Interleukin il 1β, by R&D Systems (1 mentions)

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Thrombin (13 citations)

2 protocols using thrombin antithrombin complexes tatc

Multiplex Cytokine and Coagulation Biomarker Assay

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Interleukin (IL)-6, IL-10, IL-12p70, interferon (IFN)-γ, monocyte-chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α) were measured by cytometric bead array (CBA) multiplex assay (BD Biosciences, San Jose, CA) in accordance with the manufacturers' recommendations. Thrombin-antithrombin complexes (TATc; Siemens Healthcare Diagnostics, Marburg, Germany) and D-dimer (Asserachrom D-dimer, Roche Woerden, the Netherlands) were measured with commercially available ELISA kits. Protein levels in BALF were measured using a Bradford-based protein assay (Bio-Rad Laboratories, Hercules, CA). Aspartate aminotranspherase (ASAT) and alanine aminotranspherase (ALAT) were determined with commercial available kits (Sigma-Aldrich, St. Louis, MO), using a Hitachi analyzer (Boehringer Mannheim, Mannheim, Germany) according to the manufacturers' instructions.

Kager L.M., Wiersinga W.J., Roelofs J.J., de Boer O.J., Weiler H., van 't Veer C, & van der Poll T. (2014). A Thrombomodulin Mutation that Impairs Active Protein C Generation Is Detrimental in Severe Pneumonia-Derived Gram-Negative Sepsis (Melioidosis). PLoS Neglected Tropical Diseases, 8(4), e2819.

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Measuring TFPI Activity in Mouse Plasma

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TFPI activity was measured in mouse plasma using a two-stage chromogenic TFPI assay as described previously (20 (link), 27 (link)). Standard curves were prepared by serial dilution of citrated normal mouse plasma and the mean plasma TFPI activity of WT mice was arbitrarily assigned a value of 1 U/ml. Thrombin-antithrombin complexes (TATc; Siemens Healthcare Diagnostics, Marburg, Germany), D-dimer (Asserachrom D-dimer, Roche, Woerden, the Netherlands), macrophage–inflammatory protein (MIP)–2, keratinocyte-derived cytokine (KC), interleukin (IL)-1β (R&D Systems, Abingdon, UK) and myeloperoxidase (MPO; HyCult Biotechnology, Uden, The Netherlands) were measured using commercially available ELISA kits. Tumour necrosis factor (TNF)-α, interleukin (IL)-6, interferon (IFN)-γ and monocyte chemotactic protein (MCP)-l were measured by cytometric bead array multiplex assay (BD Biosciences, San Jose, CA, USA) in accordance with the manufacturers' recommendations.

van den Boogaard F.E., Veer C.V., Roelofs J.J., Meijers J.C., Schultz M.J., Broze G J.r, & van der Poll T. (2015). Endogenous tissue factor pathway inhibitor has a limited effect on host defence in murine pneumococcal pneumonia. Thrombosis and haemostasis, 114(1), 115-122.

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