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Murine specific elisa kits

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Murine-specific ELISA kits are laboratory equipment used to detect and quantify specific analytes in mouse samples. These kits provide a standardized and reliable method for measuring target molecules in a variety of murine biological matrices.

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Cba flex set, by BD (1 mentions)

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Murine ELISA kits Specific ELISA kits Specific ELISAs ELISAs

2 protocols using murine specific elisa kits

1

Ex Vivo Quantification of Cytokine Secretion

Cited 1 time
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As described previously (35 (link)), for ex vivo quantification of cytokine secretion, isolated lamina propria lymphocytes were washed with complete tissue culture media (RPMI, 10% FBS, 1% glutamine, and 1% 1:1 penicillin/streptomycin) and cultured in 1 mL of the same media in 24-well plates for 24 h at 37 °C, 5% carbon dioxide. The resulting supernatants were used to determine the amount of each cytokine using murine-specific ELISA kits (BD Biosciences) and a cytometrix bead array (CBA) flex set (BD Biosciences) relative to a standard curve according to the manufacturer’s recommendations.
Henke M.T., Brown E.M., Cassilly C.D., Vlamakis H., Xavier R.J, & Clardy J. (2021). Capsular polysaccharide correlates with immune response to the human gut microbe Ruminococcus gnavus. Proceedings of the National Academy of Sciences of the United States of America, 118(20), e2007595118.
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2

Ex Vivo Cytokine Quantification

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For ex vivo quantification of cytokine secretion, small intestinal epithelia were washed with complete tissue culture media (RPMI, 10% FBS, 1% glutamine and 1% of 1:1 penicillin/streptomycin) and cultured in 1 mL of the same media in 24-well plates for 24 hours at 37°C, 5% carbon dioxide. The resulting supernatants were used to determine the amount of each cytokine using murine-specific ELISA kits (BD Biosciences) and a CBA flex set (BD Biosciences), relative to a standard curve, according to the manufacturer’s recommendations.
Brown E.M., Ke X., Hitchcock D., Jeanfavre S., Avila-Pacheco J., Nakata T., Arthur T.D., Fornelos N., Heim C., Franzosa E.A., Watson N., Huttenhower C., Haiser H.J., Dillow G., Graham D.B., Finlay B.B., Kostic A.D., Porter J.A., Vlamakis H., Clish C.B, & Xavier R.J. (2019). Bacteroides-derived sphingolipids are critical for maintaining intestinal homeostasis and symbiosis. Cell host & microbe, 25(5), 668-680.e7.
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