The largest database of trusted experimental protocols

Du 897u

Manufactured by Oxford Instruments

The DU-897U is a high-performance spectrophotometer designed for laboratory applications. It is capable of measuring the absorption and transmission of light across a wide range of wavelengths, making it suitable for various analytical and research tasks. The device features a robust design and advanced optics to ensure accurate and reliable measurements.

Automatically generated - may contain errors

3 protocols using du 897u

1

Super-Resolution Imaging of Brain Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
SIM on brain sections was done with a Nikon SIM system with a 100x 1.49 NA oil immersion objective, managed by NIS elements software. Tissues were imaged at laser excitation of 405 (for nuclei), 488 (for β2-GPI), 561 (for MBL-C) and 640 nm (for IB4) with a 3 D-SIM acquisition protocol. Fourteen-bit images sized 1024×1024 pixels with a single pixel of 0.030 µm were acquired in a gray level range of 0-4000 to exploit the linear range of the camera (iXon ultra DU-897U, Andor) and to avoid saturation. Raw and reconstructed images were verified by the SIMcheck ImageJ plugin.23 (link)
+ Open protocol
+ Expand
2

Super-resolution imaging of single molecules

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wafers were mounted section side down in a petri dish (glass bottom, thickness 170 μm +/−5 μm, Ibidi) onto a drop of a 1:1 mixture of glycerin (80%) and an imaging buffer containing an oxygen scavenging system (200 mM Phosphate buffer containing 10% glucose, 0.5 mg/ml glucoseoxidase, 40 μg/ml catalase, 15 mM beta-Mercaptoethylamine (MEA), pH 8.0). Excess buffer was removed until the wafer was brought as close as possible to the cover glass.
Super-resolution imaging was performed on a Leica SR-GSD 3D microscope (Leica Microsystems) using a 160x (NA 1.43) oil immersion objective (HC PL APO, Leica Microsystems). The system was equipped with 488 nm/300 mW, 532 nm/500 mW and 647 nm/500 mW continuous wave lasers and an EMCCD camera (iXon Ultra, DU-897U, Andor).
The sample was first illuminated with the 642 nm laser at maximum laser power to ensure an efficient transfer of the fluorescent molecules into the off-state. Images of 180 × 180 pixels were then taken with an integration time of 15 ms in epifluorescence mode. A total of 30,000 frames were collected for each reconstruction.
Image reconstruction and visualization via Gaussian fitting was performed with the LAS AF software (Leica Microsystems).
+ Open protocol
+ Expand
3

Single-Molecule Tensile Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
The tensile stage sits on an optical microscope. We illuminate (355 nm laser) the sample from the bottom and likewise detect phosphorescence from the bottom. For single-molecule imaging the crosslinks are diluted (phosphorescent: non-phosphorescent ~1:1000). For calibration of macroscopic force-extension relations, every crosslink was phosphorescent.
Circularly polarized light is used for best excitation efficiency, except for experiments in which we use linearly-polarized light to monitor the polarization dependence. The light emitted is recorded using an EMCCD camera (DU-897U, Andor) with a large pixel density, 512 × 512, and a ×100 oil objective (Olympus). The field of view is 80 by 80 µm, pixel size 160 nm, and depth of focus 1.5 µm. Video images are analyzed using 2D Gaussian feathering to remove background, using IDL codes written in-house, to give the absolute intensity and positions of the optical probes. To measure the phosphorescence spectrum, the tensile stage is fiber-coupled to an Ocean Optics spectrometer. Photoluminescence (PL) is quantified by integrating emission between wavelengths 500 and 700 nm.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!