Du 897u
The DU-897U is a high-performance spectrophotometer designed for laboratory applications. It is capable of measuring the absorption and transmission of light across a wide range of wavelengths, making it suitable for various analytical and research tasks. The device features a robust design and advanced optics to ensure accurate and reliable measurements.
Lab products found in correlation
3 protocols using du 897u
Super-Resolution Imaging of Brain Tissue
Super-resolution imaging of single molecules
Super-resolution imaging was performed on a Leica SR-GSD 3D microscope (Leica Microsystems) using a 160x (NA 1.43) oil immersion objective (HC PL APO, Leica Microsystems). The system was equipped with 488 nm/300 mW, 532 nm/500 mW and 647 nm/500 mW continuous wave lasers and an EMCCD camera (iXon Ultra, DU-897U, Andor).
The sample was first illuminated with the 642 nm laser at maximum laser power to ensure an efficient transfer of the fluorescent molecules into the off-state. Images of 180 × 180 pixels were then taken with an integration time of 15 ms in epifluorescence mode. A total of 30,000 frames were collected for each reconstruction.
Image reconstruction and visualization via Gaussian fitting was performed with the LAS AF software (Leica Microsystems).
Single-Molecule Tensile Microscopy
Circularly polarized light is used for best excitation efficiency, except for experiments in which we use linearly-polarized light to monitor the polarization dependence. The light emitted is recorded using an EMCCD camera (DU-897U, Andor) with a large pixel density, 512 × 512, and a ×100 oil objective (Olympus). The field of view is 80 by 80 µm, pixel size 160 nm, and depth of focus 1.5 µm. Video images are analyzed using 2D Gaussian feathering to remove background, using IDL codes written in-house, to give the absolute intensity and positions of the optical probes. To measure the phosphorescence spectrum, the tensile stage is fiber-coupled to an Ocean Optics spectrometer. Photoluminescence (PL) is quantified by integrating emission between wavelengths 500 and 700 nm.
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