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2 protocols using rhodamine b

1

Synthesis and Characterization of COVID-19 Proteins

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HAuCl4·3H2O (99.9% pure), H2PtCl6·6H2O (99% pure), L-(+)-ascorbic acid (99% pure), 3,3′-diaminobenzidine (99% pure), o-phenylenediamine (98% pure) and carbamoyl-2,2,5,5-tetramethyl-3-pyrrolin-1-yloxy (CTPO, 99% pure) were provided by Alfa Aesar. 5,5-Dimethyl-1-pyrroline N-oxide (DMPO, 98% pure) and 2,2,6,6-tetramethylpiperidine (TEMP, 99% pure) were acquired from Innochem. Rhodamine B and glucose oxidase (GOx) were supplied by Amresco. Reduced cytochrome C was purchased from Abcam (Shanghai, China). 2,2′-Azinobis (3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (99% pure) was bought from J&K Scientific Ltd. (Beijing, China). TMB (99% pure) was obtained from Acros. Trisodium citrate dihydrate (99% pure) was provided by Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). PBS buffer (pH 7.2–7.4), α-amylase, lysozyme, and horseradish peroxidase (HRP) were provided by Solarbio Science & Technology Co., Ltd. (Beijing, China). Collagen was supplied by Yuanye Co., Ltd. (Shanghai, China). SARS-CoV-2 S1 recombinant protein (S1 protein), nucleocapsid protein (N protein) and anti-SARS-CoV-2 S1 antibody were acquired from Sangon Biotech Co., Ltd. (Shanghai, China).
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2

Synthesis and Characterization of Silica Nanoparticles

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Sodium alginate was purchased from Acros (New Jersey, USA); tetraethyl orthosilicate (98% purity) was purchased from Sigma Aldrich (Missouri, USA); Triton X-100 and rhodamine B were purchased from AMRESCO (Ohio, USA); cyclohexane, ethanol, aqueous ammonia solution (29 wt.% ammonia), isopropyl alcohol (70%), hydrochloric acid (37%), and sulfuric acid were purchased from BDH Chemicals (Pennsylvania, USA). Sodium alginate (very low viscosity) and n-hexanol, were purchased from Alfa Aesar (Massachusetts, USA). All chemicals were used as provided without further purification. Deionized (DI) water was obtained from an Elga Purelab Flex water purification system (Veolia Water Technologies, UK).
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