Golgin 97

Unless otherwise mentioned reagents were from Sigma-Aldrich (St. Louis, MO, USA) and were of the highest analytical grade. Antibodies against AMPK, phospho-Thr172-AMPK, LC3B, GAPDH, Golgin 97, Rab7, PARP, phospho-Ser780 pRb, and phospho-Ser15 p53 were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies against V-ATPase B2 subunit, E-cadherin, p21, and LAMP1 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and antibody against β-actin was from Sigma-Aldrich. Antibodies against alpha-adaptin 2, MT1-MMP (MMP14) and p62 were from Abcam (Cambridge, UK). HIF-1α and p150^Glued antibodies were from BD Biosciences (San Jose, CA, USA). Antibodies against Giantin and HSP47 were from Enzo Lifesciences (Farmingdale, NY, USA). HRP-conjugated secondary antibodies for Western blotting (goat-anti-mouse (GAM) and goat-anti-rabbit (GAR)) were from DAKO (Glostrup, Denmark). Rhodamin phalloidin, Alexa Fluor 568 conjugated GAM, and Alexa Fluor 488 conjugated GAR secondary antibodies for immunofluorescence were from Invitrogen (Carlsbad, CA, USA). Antibody against Cortactin was from Merck (Darmstadt, Germany). Concanamycin A, Forskolin, and antibody against TCIRG1 (V-ATPase a3 subunit) were from Sigma-Aldrich (St. Louis, MO, USA).

Golgin 97 and Pan-Cadherin from Cell Signalling Technology. Calreticulin from Genetex. Py99, FGFR2, Flotillin-1, GRB2 antibodies from Santa Cruz. c-CBL from ThermoScientific. Lipid standards were from Larodan, Sweden. Solutes for HPTLC were from Sigma.