SH‐SY5Y cells were obtained from ATCC. All cells were cultured in DMEM supplemented with 10% FBS and non‐essential amino acids at 37°C in a humidified incubator containing 5% CO2. The BCL‐2, BCL‐xL, AIF and BAX antibodies were obtained from Abcam. Caspase‐3 antibody (9662S) was obtained from Cell Signaling Technology. GAPDH and β‐actin antibodies were obtained from Proteintech. The LDH cytotoxicity assay kit (J2380) was obtained from Promega. ATP Cell Titer‐Glo assay kit was obtained from Promega. The Mitoview 633 and DAPI were obtained from Biotium.
Caspase 3 antibody 9662
Manufactured by Cell Signaling Technology
Sourced in United States
Caspase-3 antibody (9662S) is a laboratory reagent used to detect the presence and relative abundance of caspase-3 protein in biological samples. Caspase-3 is a key enzyme involved in the execution phase of cell apoptosis, or programmed cell death. This antibody can be used in various immunodetection techniques, such as Western blotting, to study the role of caspase-3 in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Bcl 2, by Abcam (1 mentions)
Bcl2 associated x (bax), by Abcam (1 mentions)
Sh sy5y cell, by American Type Culture Collection (1 mentions)
Bcl xl, by Abcam (1 mentions)
Mitoview 633, by Biotium (1 mentions)
Ldh cytotoxicity assay kit, by Promega (1 mentions)
U251 cells, by American Type Culture Collection (1 mentions)
Radio immunoprecipitation assay ripa lysis buffer, by Beyotime (1 mentions)
Palmitate, by Merck Group (1 mentions)
4 2 aminoethyl benzenesulfonyl fluoride hydrochloride aebsf, by Merck Group (1 mentions)
β actin antibody sc 1616, by Santa Cruz Biotechnology (1 mentions)
Asym24 antibody, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Ham s f 12 nutrient mixture, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Thapsigargin, by Merck Group (1 mentions)
Gsk2606414, by Merck Group (1 mentions)
4 protocols using caspase 3 antibody 9662
1
Molecular Apoptosis Mechanisms in SH‐SY5Y Cells
2
Culturing U251 Glioblastoma Cells
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U251 cells were obtained from ATCC. All cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and non-essential amino acids, 100 IU penicillin and 100 mg/mL streptomycin at 37 °C in a humidified incubator containing 5% CO2. GSDME antibody (ab215191) was obtained from Abcam. Caspase-3 antibody (9662S) was from Cell Signaling Technology. LaminB1 antibody (66095-1-Ig) was from Proteintech.
3
Protein Expression Analysis in Ischemic Brain
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Proteins were extracted from both ischemic and control brain cortices using Radio-Immunoprecipitation Assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology, Shanghai, China). Equal proteins were resolved on 10–12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel and then transferred onto polyvinylidene fluoride (PVDF) membranes. After blocking, the membrane was incubated with the following primary antibodies (Cell Signaling, San Jose, CA, USA) at 4 °C overnight: Caspase-3 Antibody #9662, CD54/ICAM-1 (E3Q9N) XP® Rabbit mAb#67836, NRF-2 (D1Z9C) XP® Rabbit mAb#12721, HO-1 (E3F4S) Rabbit mAb #43966 and β-Actin (D6A8) Rabbit mAb #8457. Subsequently, anti-rabbit IgG, HRP-linked Antibody #7074 (Cell Signaling) was added for an incubation time of 1 hour. The proteins were detected using a ChemiDoc XRS imaging system, and image J software was employed to analyze the relative density of protein bands.
4
Palmitate-induced ER stress signaling
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Dulbecco’s Modified Eagle’s Medium (DMEM), Ham’s nutrient mixture F-12, and fetal bovine serum (FBS) were purchased from Life Technologies (Gibco BRL, Grand Island, NY, USA). Palmitate and 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The Palmitate was prepared as described previously [30 (link)]. Thapsigargin and GSK2606414 were purchased from Millipore (Billerica, MA, USA). p-IRE antibody (ab48187), ATF6 antibody (ab11909), PRMT1 antibody (ab3768), and PRMT4 antibody (ab110024) were purchased from Abcam (Cambridge, UK). caspase-3 antibody (#9662), p-eIF2α antibody (#9721), and CHOP antibody (#2895) were purchased from Cell Signaling Technology (Beverly, MA, USA). ASYM24 antibody (#07-414) was obtained from Millipore. β-actin antibody (sc-1616) and p-PERK antibody (sc-32577-R) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All reagents were of the highest purity commercially available.
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