Rpmi culture medium

RPMI culture medium was purchased from Corning (Corning, NY). Fetal bovine serum (FBS) was purchased from Sigma Chemical Co. (St. Louis, MO). Trypsin, pen/strep antibiotics, and puromycin were purchased from Gibco (Grand Island, NY). Trametinib (MEK inhibitor) was purchased from Selleckchem (Houston, TX). Antibodies for Western Blot: Anti-HDAC1 (#2062, from Cell Signaling Technology (Danvers, MA), anti-HDAC2 (#2540 CST), The anti-HDAC3 and anti-HDAC8 antibodies were described in refs. 17 (link), HDAC11(#58442, CST). Anti-HDAC6 (#H-300, sc-11420) was purchased from Santa Cruz Biotechnologies. Cleaved PARP (Asp214) (#D64E10) (#5625 CST), phospho LKB1 (#3482, CST), total LKB1 (#3050, CST). Anti-YAP1 antibody (#H00010413-M01, Abnova). Anti-Vinculin (#G8796) and anti-GAPDH (#V9131) were purchased from Millipore Sigma (Bedford, MA).

RPMI culture medium was purchased from Corning (Corning, NY). Fetal bovine serum (FBS) was purchased from Sigma Chemical Co. (St. Louis, MO). Trypsin, pen/strep antibiotics, and puromycin were purchased from Gibco (Grand Island, NY). Trametinib (MEK inhibitor), Panobinostat (pan-HDAC inhibitor), Pictilisib (PI3K inhibitor), Bosentan Hydrate (EDNRB inhibitor), Verteporfin (YAP inhibitor), Entinostat (HDAC1/2/3 inhibitor), and Tubastatin A (HDAC 6 inhibitor) were purchased from Selleckchem (Houston, TX). PCI-34051 (HDAC8 inhibitor) was purchased from Cayman Chemical (Ann Arbor, MI). Endothelin-3 was purchased from Sigma Chemical Co. (St. Louis, MO). WNT5A was purchased from R&D Systems (Minneapolis, MN, USA). Antibodies for Western Blot and immunochemistry were purchased from Cell Signaling Technology (Danvers, MA), Sigma Chemical Co. (St. Louis, MO), Millipore (Bedford, MA) and Abcam (Cambridge, MA). The phospho-Receptor Tyrosine Kinase and phospho-Kinase array were purchased from R&D Systems (Minneapolis, MN, USA). Opti‐MEM medium, Lipofectamine 2000 and Live/Dead viability stain were purchased from Invitrogen/Life Technologies Corp). siRNA for ROR1/2, IGF-1R and YAP were purchased from Dharmacon RNA Technologies (Lafayette, CO). Nontargeting siRNA was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). The Endothelin-3 Assay Kit was purchased from IBL (Takasaki, Japan).