Sheep anti tyrosine hydroxylase

The following antibodies were used at the indicated dilutions: rabbit anti-GFP (1:500, Millipore, Darmstadt, Germany, RRID:AB_91337), mouse anti-DSCAM (1:50, R&D Systems clone 36661, RRID:AB_2095452), sheep anti-tyrosine hydroxylase (1:500, Millipore, RRID:AB_11213126), rabbit anti-bNOS (1:500, Sigma RRID:AB_260796), rabbit anti-melanopsin (1:10,000 gift of Dr. Ignacio Provencio at the University of Virginia or Advanced Targeting Systems, San Diego, CA, RRID:AB_1266795), rabbit anti-Dab1 (1:500, Millipore, RRID:AB_2261451), guinea-pig anti-VGLUT3 (1:10,000, Millipore, RRID:AB_2187832), rabbit anti-PKCa (1:1000, Sigma RRID:AB_477345), goat anti-ChAT (1:400, Millipore, RRID:AB_2079751), rabbit anti-HA tag (1:250, Sigma, RRID:AB_260070). All secondary antibodies were alexa-fluor conjugates (1:500, Thermo Fisher ).

Primary antibodies used included the following: Guinea pig anti-VGLUT3 (1:10,000, Millipore), sheep anti-tyrosine hydroxylase (1:500, Millipore), rabbit anti-melanopsin (1:10,000, ATS), mouse anti-Brn3a (1:500, Millipore), goat anti-choline acetyltransferase (1:500, Millipore), mouse anti-NeuN (1:500, Millipore), mouse anti-GFAP (1:500, Sigma), rabbit anti-FoxP2 (1:4000, Abcam), rabbit anti-Pax2 (1:200, Zymed), rabbit anti-cleaved caspase 3 (1:100, Cell Signaling Technologies), mouse anti-parvalbumin (1:500, Sigma), and mouse anti-GAPDH (1:500, Abcam). Mouse monoclonal antibodies against γ-Pcdh proteins used for western blots (1:500–1:1000) were generated by NeuroMab in collaboration with the Weiner laboratory [55 (link)] and obtained from Antibodies, Inc.: N159/5 (detecting an epitope in constant exon 1 or 2 and thus all 22 γ-Pcdh isoforms); N144/32 (detecting all γA subfamily isoforms); N148/30 (specific for γB2); N174B/27 (specific for γC3). A rabbit polyclonal antibody raised at Affinity BioReagents against the peptide sequence VAGEVNQRHFRVDLD (within EC1) from murine γC4 was also used for western blotting (1:1000). Secondary antibodies were conjugated with Alexa-488, -568, or -647 (1:500, Invitrogen) or HRP (1:1000–1:5000, Jackson Immunoresearch).