Total DNA was extracted from the bacterial DNA extraction as described by Seldin and Dubnau [42 (link)] using phenol-chloroform. After extraction, the DNA sample was mixed with blue juice dye in a proportion of 1:2 of the total volume of the mixture and subjected to horizontal electrophoresis in 1 % (w/v) agarose gel. Ethidium bromide (0.1%) was added to 10× diluted Tris-acetate-EDTA (TAE) buffer for 30 min at a constant voltage of 90 V. The molecular weight marker used was the “1 Kb Plus DNA Ladder” (Promega®, Madison, USA). The gel was observed and photographed under UV light using a UV light transilluminator (model) to analyze the purity and quality of the genetic material.
1 kb plus dna ladder
Manufactured by Promega
Sourced in United States
The 1 Kb Plus DNA Ladder is a pre-stained DNA molecular weight marker used for estimating the size of DNA fragments in agarose gel electrophoresis. It contains DNA fragments ranging from 100 base pairs to 12 kilobase pairs, providing a wide size range for DNA size determination.
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Lab products found in correlation
3 protocols using 1 kb plus dna ladder
1
Bacterial DNA Extraction and Electrophoresis
2
Anaplasma 16S rRNA Gene PCR
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Of the extracted DNA, 2.0ul was used as template in the PCR reaction, targeting a 345 bp fragment of the Anaplasma 16S rRNA gene, using the EHR16SD/R primer set and previously defined conditions [21 (link)]. Amplicons were visualized on 1% agarose gels to validate amplicon size by comparison with a DNA molecular weight marker (1 Kb Plus DNA Ladder, Promega, Madison, USA).
3
Anaplasma 16S rRNA Gene Amplification
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The primer set EHR16SD/R was used in a simple PCR to amplify 345bp fragment of Anaplasma 16S rRNA gene on the same samples with thermo-cycling profile as previously described [24] (link). PCR products were electrophoresed on 1% agarose gel to check the size of amplified fragments by comparison with a DNA molecular weight marker (1 Kb Plus DNA Ladder, Promega, Madison, USA).
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