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Jmn eca600

Manufactured by JEOL
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Sourced in Japan

The JMN-ECA600 is an electron microscope designed for materials analysis. It provides high-resolution imaging and analytical capabilities for a variety of sample types. The instrument features an advanced electron optics system and various detectors to enable comprehensive materials characterization.

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Lab products found in correlation

Jmn ecs400, by JEOL (6 mentions) P 2200, by Jasco (5 mentions) C18 cartridges, by Biotage (4 mentions) J 820 spectrometer, by Jasco (4 mentions) Rp 18 f254, by Merck Group (3 mentions) Ftir 8700, by Shimadzu (3 mentions) Nicolet is5 ft tr spectrometer, by Thermo Fisher Scientific (2 mentions) Combiflash rf, by Teledyne (2 mentions) P 2200 digital polarimeter, by Jasco (2 mentions) Silica gel 60n, by Kanto Chemical (2 mentions) Rp 18f254 plates, by Merck Group (2 mentions) R axis rapid 2, by Rigaku (1 mentions) Silica gel 60 rp 18 f254s, by Merck Group (1 mentions) Jms t100td, by JEOL (1 mentions) Single reflection atr accessory, by Shimadzu (1 mentions) Irspirit ftir spectrometer, by Shimadzu (1 mentions) Sf r c18 d, by Biotage (1 mentions) Inertsustain c18 column, by GL Sciences (1 mentions) Ecs 400, by JEOL (1 mentions) Silica gel 60 f254 plate, by Merck Group (1 mentions)

8 protocols using jmn eca600

1

Spectroscopic Characterization of Compounds

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Optical rotations were measured on a JASCO P-2200 digital polarimeter. CD spectra were recorded on JASCO J-820 spectrometer. Infrared spectra (IR) were obtained with a Thermo Fisher Scientific NICOLET iS5 FT-TR spectrometer from samples in CH2Cl2. NMR spectra were measured on JEOL JMN-ECA600 and JMN-ECS400 spectrometers with tetramethylsilane as an internal standard, and chemical shifts are stated as δ values. HRMS data were recorded on a JMS-700 MStation quadrupole (FAB) or JMS-T100TD TOF (DART) mass spectrometer. A crystal of the compound was measured on a R-AXIS RAPID II (Rigaku). Analytical and preparative TLC were carried out on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed on Combiflash Rf (Teledyne Isco) with silica gel and C18 cartridges (Biotage, Uppsala Sweden). Preparative HPLC was operated on a GL Science recycling system using an InertSustain C18 column (5 μm, 20 × 250 mm).
Aimaiti S., Suzuki A., Saito Y., Fukuyoshi S., Goto M., Miyake K., Newman D.J., O’Keefe B.R., Lee K.H, & Nakagawa-Goto K. (2018). Corymbulosins I−W, Cytotoxic Clerodane Diterpenes from the Bark of Laetia corymbulosa. The Journal of organic chemistry, 83(2), 951-963.
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2

Analytical Methods for Compound Characterization

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Optical rotations were determined on a JASCO P-2200 digital polarimeter. ECD spectra were measured on a JASCO J-820 spectrometer. Infrared spectra (IR) were recorded with a Thermo Fisher Scientific NICOLET iS5 FT-TR spectrometer with samples in CH2Cl2. NMR spectra were obtained on JEOL JMN-ECA600 and JMN-ECS400 NMR spectrometers with tetramethylsilane as an internal standard, and chemical shifts are stated as δ values. HRMS data were recorded on a JMS-SX102A (FAB) or JMS-T100TD (DART) mass spectrometer. Analytical and preparative TLC were performed on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed on a Combiflash Rf (Teledyne Isco) instrument with silica gel and C18 cartridges (ODS-25, YMC-DispoPack). Preparative HPLC was conducted on a GL Science recycling system using an InertSustain C18 column (5 µM, 20 × 250 mm).
Suzuki A., Saito Y., Fukuyoshi S., Goto M., Miyake K., Newman D.J., O’Keefe B.R., Lee K.H, & Nakagawa-Goto K. (2017). Corymbulosins D–H, 2-Hydroxy- and 2-Oxo-clerodane Diterpenes from the Bark of Laetia corymbulosa. Journal of natural products, 80(4), 1065-1072.
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3

Analytical Techniques for Chemical Structure Elucidation

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A JASCO P-2200 digital polarimeter was used for the determination of optical rotations. ECD spectra were detected on a JASCO J-820 spectrometer. NMR spectra were taken on JEOL JMN-ECA600 and JMN-ECS400 NMR spectrometers. Tetramethylsilane was used as an internal standard, and chemical shifts are stated as δ values. HRMS data were obtained on a JMS-SX102A (FAB) or JMS-T100TD (DART) mass spectrometer. Analytical and preparative TLC were conducted on RP-18F254 plates and precoated silica gel 60F254 (0.50 or 0.25 mm thickness; Merck). Column chromatography was performed with silica gel 60 N (63–210 μm, spherical, neutral, Kanto Chemical). Silica gel 60 RP-18 F254S (0.25 mm, Merck) was applied for reversed-phase and 60 F254 (1 mm) for normal-phase preparative TLC.
Rahim A., Saito Y., Miyake K., Goto M., Chen C.H., Alam G., Morris-Natschke S., Lee K.H, & Nakagawa-Goto K. (2018). Kleinhospitine E and Cycloartane Triterpenoids from Kleinhovia hospita. Journal of natural products, 81(7), 1619-1627.
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4

Spectroscopic Characterization of Organic Compounds

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Optical rotations were recorded on a JASCO P-2200 digital polarimeter. Infrared spectra (IR) were measured with a Shimadzu FTIR-8700 instrument for samples in CHCl3. NMR spectra were taken on a JEOL JMN-ECA600 spectrometer and JMN-ECS400 spectrometer with tetramethylsilane as an internal standard, and chemical shifts are expressed as δ values. High-resolution mass spectrometric data were obtained on a JEOL JMS-700 (FAB) or a JMS-T100TD (DART) mass spectrometer. Analytical and preparative TLC was carried out on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed with silica gel and C18 cartridges (Biotage, Uppsala, Sweden).
Miyake K., Morita C., Suzuki A., Matsushita N., Saito Y., Goto M., Newman D.J., O’Keefe B.R., Lee K.H, & Nakagawa-Goto K. (2019). Prenylated Acetophloroglucinol Dimers from Acronychia trifoliolata: Structure Elucidation and Total Synthesis. Journal of natural products, 82(10), 2852-2858.
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5

Characterization of Synthetic Compounds

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Optical rotations were recorded on a JASCO P-2200 digital polarimeter. Infrared spectra (IR) were measured with a Shimadzu FTIR-8700 instrument for samples in CHCl3. NMR spectra were acquired on JEOL JMN-ECA600 and JMN-ECS400 spectrometers with tetramethylsilane as internal standard, and chemical shifts are expressed as δ values. HRMS data were obtained on a JMS-SX102A (FAB) or JMS-T100TD (DART) mass spectrometer. Microwave irradiation experiments were carried out in a dedicated Biotage Initiator 2.5 microwave apparatus. Analytical and preparative TLC was carried out on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed with silica gel and C18 cartridges (Biotage, Uppsala Sweden). Compounds 9–1316 ,17 (link),20 and 1921 (link) were obtained previously.
Morita C., Kobayashi Y., Saito Y., Miyake K., Tokuda H., Suzuki N., Ichiishi E., Lee K.H, & Nakagawa-Goto K. (2016). Total Synthesis and in Vitro Anti-Tumor-Promoting Activities of Racemic Acetophenone Monomers from Acronychia trifoliolata. Journal of natural products, 79(11), 2890-2897.
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6

Characterization of Organic Compounds

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Optical rotations were recorded on a JASCO P-2200 digital polarimeter. Infrared spectra (IR) were measured with a Shimadzu FTIR-8700 instrument for samples in CHCl3. NMR spectra were recorded on JEOL JMN-ECA600 and JMN-ECS400 spectrometers with tetramethylsilane as an internal standard, and chemical shifts are expressed as δ values. HRMS data were obtained on a JMS-SX102A (FAB) or JMS-T100TD (DART) mass spectrometer. Analytical and preparative TLC were carried out on precoated silica gel 60F254 and RP-18F254 plates (0.25 or 0.50 mm thickness; Merck). MPLC was performed with silica gel and C18 cartridges (Biotage, Uppsala Sweden).
Miyake K., Suzuki A., Morita C., Goto M., Newman D.J., O’Keefe B.R., Morris-Natschke S.L., Lee K.H, & Nakagawa-Goto K. (2016). Acetophenone Monomers from Acronychia trifoliolata. Journal of natural products, 79(11), 2883-2889.
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7

Spectroscopic Characterization of Compounds

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Optical rotations were determined using a JASCO P-2200 digital polarimeter (JASCO, Tokyo, Japan). The UV spectra were measured with a Thermo Scientific Genesys 50 spectrometer (Thermo Scientific, Waltham, MA, USA). ECD spectra were measured with a JASCO J-820 spectrometer. Infrared spectra (IR) were recorded with a Shimadzu IRSpirit FT-IR spectrometer (Shimadzu, Kyoto, Japan) with a QATR-S single reflection ATR accessory, using neat samples. The NMR spectra were obtained via the JEOL JMN-ECA600 and JMN-ECS400 NMR spectrometers (JEOL, Akishima, Japan), with tetramethylsilane as an internal standard; chemical shifts are stated as δ values. HRMS data were recorded on a JMS-700 MStation (FAB) mass spectrometer. MPLC analysis was performed with C18 cartridges (Sfär C18 D, Biotage, Charlotte, NC, USA). Preparative HPLC analysis was conducted on a GL Science recycling system using an InertSustain C18 column (5 μm; 20 × 250 mm) (GL Sciences, Tokyo, Japan). LC/MS analysis was recorded with a Shimadzu LCMS-9030 connected to an Imtakt Cadenza CX-C18 (2 × 50 mm; 3 μm) (Imtakt, Kyoto, Japan).
Amuti S., Saito Y., Fukuyoshi S., Miyake K., Newman D.J., O’Keefe B.R., Lee K.H, & Nakagawa-Goto K. (2024). Unusual Vilasinin-Class Limonoids from Trichilia rubescens. Molecules, 29(3), 651.
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8

NMR Spectroscopy and Chromatography Protocol

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NMR spectra were measured on JEOL JMN-ECA600 and JMN-ECS400 spectrometers with tetramethylsilane as an internal standard and CDCl3 as solvent for both 1 H and 13 C NMR, and chemical shifts are stated as δ values. Analytical and preparative thin-layer chromatography (TLC) were performed using precoated silica gel 60 F254 plates (0.25 mm, Merck) for normal-phase and silica gel 60 RP-18 F254S plates (0.25 mm, Merck) for reversedphase. Column chromatography was carried out with silica gel 60 N (Spherical, 63-210 µm, neutral, Kanto Chemical).
Astuti A.D., Yasir B., Rahim A., Natzir R., Subehan S., Nakagawa‐Goto K., & Alam G. (2021). Isolation and Characterization of Stigmasterol and β-sitosterol from Plectranthus scutellarioides var. color blaze dark star and Cytotoxicity of Its Fraction. Egyptian Journal of Chemistry, 0(0), 0-0.
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