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2 protocols using anti phosphor stat3 tyr705

1

Antibody Detection Protocol for Cellular Signaling

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Anti-CD40 (#28158-1-AP, 1 : 1000), anti-AKT (#10176-2-AP, 1 : 1000), anti-GAPDH (#10494-1-AP, 1 : 10000), and anti-ERK (#16443-1-AP, 1 : 1000) antibodies were purchased from Proteintech (Hubei, Wuhan, China). anti-AKT-phosphor-Ser473 (#4060, 1 : 1000) and NF-κB p65 (#8242, 1 : 1000) antibodies were obtained from Cell Signaling Technology (Massachusetts, USA). Anti-phosphor-ERK (#461212082, 1 : 1000) was bought from Sigma (St. Louis, MO, USA). Anti-phosphor-STAT3-Tyr705 (#abs130918, 1 : 1000) was bought from absin (China). And anti-STAT3 (#GB11176, 1 : 1000) and anti-ICAM-1 (#GB11106, 1 : 1000) were obtained from Servicebio (Wuhan, China).
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2

Modulation of Autophagy by 3-Methyladenine and Betulinaldehyde

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3-Methyladenine (Cat No: HY-19312) and Betulinaldehyde (Cat No: HY-N0084) were purchased from MCE (Shanghai, China). The rAd-mCherry-GFP-LC3B was obtained from Servicebio (Wuhan, China). The anti-AKT (Cat No: 10176-2-AP, 1:1000), anti-AKT-phosphor-Ser473 (Cat No: 66444-1-Ig, 1:3000), anti-SQSTM1 (Cat No: 18420-1-AP, 1:2000), anti-actin (Cat No: 66009-1-Ig, 1:5000), HRP goat anti-Mouse IgG (Cat No: SA00001-1, 1:5000), HRP goat anti-rabbit IgG (Cat No: SA00001-1, 1:6000) were purchased from Proteintech (Hubei, Wuhan, China). The anti-ERK (Cat No: ab184699, 1:10000) was purchased from abcam (Cambridge, UK). Anti-phosphor-ERK (Cat No: AF1015, 1:1000) was purchased from affbiotech (Cincinnati, OH, USA). The anti-LC3 (Cat No: 12741S, 1:1000) was purchased from Cell Signaling Technology (Massachusetts, USA). Anti-phosphor-STAT3-Tyr705 (Cat No: abs130918, 1:1000) was bought from absin (China). And anti-STAT3 (Cat No: GB11176, 1:1000) was obtained from Servicebio (Wuhan, China).
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