N. khasiana leaves and pitchers were dried at 60 °C for 72 h and their carbon and nitrogen contents were analyzed on a Vario EL III CHN Analyzer (Elementar Analysensysteme GmbH, Hanau, Germany).
Vario el 3 chn analyzer
Manufactured by Elementar
Sourced in Germany
The Vario EL III CHN Analyzer is a laboratory instrument designed for the determination of carbon, hydrogen, and nitrogen in solid and liquid samples. It utilizes combustion analysis to precisely measure the elemental composition of a wide range of materials.
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3 protocols using vario el 3 chn analyzer
1
Analyzing Carbon and Nitrogen Content
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N. khasiana leaves and pitchers were dried at 60 °C for 72 h and their carbon and nitrogen contents were analyzed on a Vario EL III CHN Analyzer (Elementar Analysensysteme GmbH, Hanau, Germany).
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N. khasiana leaves and pitchers were dried at 60 °C for 72 h and their carbon and nitrogen contents were analyzed on a Vario EL III CHN Analyzer (Elementar Analysensysteme GmbH, Hanau, Germany).
2
Soil Characterization and Climate Data Analysis
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Supplementary weather data was gained from the German Meteorological Service (DWD, www.dwd.de/cdc ). Data for rainfall and average air temperature were used from the closest weather station in Görmar (station ID 6305, geographic location at N 51.2065 and E 10.4948). The daily values were recalculated to the average (temperature) or sum (rainfall) of 5 days, 10 days, and 4 weeks before sampling day.
The gravimetric water content of the soil samples was analyzed by oven drying the samples at 105°C for ≥24 h or until constant weight. The pH was determined in duplicate, using a glass electrode (WTW pH electrode SenTix 41, Xylem Analytics Germany Sales GmbH & Co. KG, Weilheim, Germany) in the supernatant of soil suspensions prepared using 1:2.5 mixtures of soil and 0.01 M CaCl2.
Before chemical analyses, visible plant residues and stones were removed by hand from the air-dried soil samples, and the samples were finely grounded to a size of 100 μm. Total carbon and total nitrogen contents of the soil samples were determined by dry combustion of triplicate sub-samples using a Vario El III C/H/N analyzer (Elementar, Hanau, Germany).
The gravimetric water content of the soil samples was analyzed by oven drying the samples at 105°C for ≥24 h or until constant weight. The pH was determined in duplicate, using a glass electrode (WTW pH electrode SenTix 41, Xylem Analytics Germany Sales GmbH & Co. KG, Weilheim, Germany) in the supernatant of soil suspensions prepared using 1:2.5 mixtures of soil and 0.01 M CaCl2.
Before chemical analyses, visible plant residues and stones were removed by hand from the air-dried soil samples, and the samples were finely grounded to a size of 100 μm. Total carbon and total nitrogen contents of the soil samples were determined by dry combustion of triplicate sub-samples using a Vario El III C/H/N analyzer (Elementar, Hanau, Germany).
3
Synthesis and Characterization of a Novel Heterocyclic Compound
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1-(2- aminophenyl) pyrrole and 5-nitro-2- thiophene carboxaldehyde were purchased from Sigma Aldrich. All the chemicals and reagents were of analytical grade and used without further purification. Electronic spectra of the compound were recorded in DMF on a Thermo electron Nicolet evolution 300 UV–Vis spectrophotometer. FT-IR spectra of the compound were recorded as KBr pellets with a JASCO-8000 FT-IR spectrophotometer in the 400–4000 cm−1 range. Elemental analyzes of the compound were done using an Elementar Vario EL III CHN analyzer at Sophisticated Test and Instrumentation Centre (SAIF), Cochin University of Science and Technology, Kochi, India. 1H and 13C NMR spectra were recorded in CDCl3 on a Burker Advance DRX 300 FT-NMR spectrometer with TMS as the internal standard.
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