Hilyte fluor 555 labeled aβ 1 42

Manufactured by AnaSpec

HiLyte Fluor 555-labeled Aβ(1–42) is a synthetic peptide with the amyloid-beta sequence (1-42) labeled with the fluorescent dye HiLyte Fluor 555. This peptide is used as a tool for research and analysis purposes.

Automatically generated - may contain errors

Lab products found in correlation

Rolipram, by Merck Group (1 mentions) Thiazolyl blue tetrazolium bromide, by Merck Group (1 mentions) Dibutyryl camp, by Merck Group (1 mentions) Isobutylmethylxanthine, by Merck Group (1 mentions) Forskolin, by Merck Group (1 mentions) Kt5720, by Merck Group (1 mentions) Sq22536, by Merck Group (1 mentions) Esi 09, by Merck Group (1 mentions)

Spelling variants of this product

HiLyte Fluor‐555‐labeled (2 citations)

2 protocols using hilyte fluor 555 labeled aβ 1 42

Amyloid-β Aggregation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aβ(1–42).HFIP (human), Aβ(42–1), and HiLyte Fluor 555-labeled Aβ(1–42) were from AnaSpec. Dibutyryl-cAMP, forskolin, rolipram, isobutylmethyl-xanthine (IBMX), and thiazolyl blue tetrazolium bromide (MTT) were obtained from Sigma-Aldrich. PACAP(1–38) peptide was from Abbiotec. SQ 22536, KT5720, and ESI-09 were from EMD Millipore. Anti-67LR mouse monoclonal antibody (clone MluC5), Alexa Fluor 488-labeled anti-67LR mouse monoclonal antibody (H-2), and mouse IgM were from Santa Cruz Biotechnology. Alexa Fluor 647-labeled anti-CD230 (prion) mouse monoclonal antibody (808008) and Alexa Fluor 594-labeled anti-Aβ mouse monoclonal antibody (800716) were from BioLegend.

Gopalakrishna R., Lin C.Y., Oh A., Le C., Yang S., Hicks A., Kindy M.S., Mack W.J, & Bhat N.R. (2022). Decrease in cell-surface laminin receptor and cellular prion protein by cAMP attenuates amyloid-β uptake and amyloid-β-induced neuronal cell death. FEBS letters, 596(22), 2914-2927.

+ Open protocol

+ Expand

Aβ Uptake in Astrocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Control (GFP) or Igfr knockout (CRE) astrocytes in culture were treated with 0.1 μM of human HiLyte™ Fluor 555 – labeled Aβ (1–42) (Anaspec, Fremont, CA) for 4 h in serum free media as previously described [43] (link). Following treatment, astrocytes were rinsed twice with 1X HBSS without calcium and magnesium, harvested with 0.25% trypsin, and spun at 1000 × g for 10 min. The pelleted cells were re-suspended in 1X HBSS with 0.1% BSA. For imaging, cells on coverslips were fixed and processed as described above in “Immunocytochemistry” methods.

Logan S., Pharaoh G.A., Marlin M.C., Masser D.R., Matsuzaki S., Wronowski B., Yeganeh A., Parks E.E., Premkumar P., Farley J.A., Owen D.B., Humphries K.M., Kinter M., Freeman W.M., Szweda L.I., Van Remmen H, & Sonntag W.E. (2018). Insulin-like growth factor receptor signaling regulates working memory, mitochondrial metabolism, and amyloid-β uptake in astrocytes. Molecular Metabolism, 9, 141-155.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!