To determine oligo-isoprene aldehydes and acids, an enzymatic reaction mixture was extracted with pentane, dried in vacuo, and dissolved in 2 mL of methanol. Then, 5 μL of the extract was subjected to a liquid chromatography-mass spectrometry (LC–MS) system (Infinity Lab LC/MSD; Agilent Technology Inc., Santa Clara, CA, USA) equipped with a ZORBAX SB-C18 2.1 × 50 mm column (Agilent Technology). LC–MS analysis was. performed as described previously [16 (link)].
Infinitylab lc msd
Manufactured by Agilent Technologies
Sourced in United States
The InfinityLab LC/MSD is a liquid chromatography-mass spectrometry (LC/MS) system designed for analytical applications. It combines liquid chromatography capabilities with mass spectrometry detection to enable the separation, identification, and quantification of chemical compounds in complex samples.
Automatically generated - may contain errors
Lab products found in correlation
Agilent 1260 infinity 2, by Agilent Technologies (1 mentions)
1260 infinity 2 lc system, by Agilent Technologies (1 mentions)
Mestrenova, by Mestrelab Research (1 mentions)
1260 infinity 2, by Agilent Technologies (1 mentions)
Coelenterazine, by GoldBio (1 mentions)
Avance 3 hdnanobay nmr spectrometer, by Bruker (1 mentions)
Agilent 1260 hplc, by Agilent Technologies (1 mentions)
Avance 3 hd 400 spectrometer, by Bruker (1 mentions)
Discovery c18, by Waters Corporation (1 mentions)
6 protocols using infinitylab lc msd
1
Quantification of Oligo-Isoprene Metabolites
2
LC-MS Analysis of Puerarin Palmitate
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LC–MS was performed using Agilent 1260 Infinity II and Infinity Lab LC/MSD (Agilent Technologies). The type of column used in the analysis, the injection volume, and the column temperature were all maintained the same as for the HPLC analysis. For the mobile phase, 0.01% formic acid in water (A) and 0.01% formic acid in acetonitrile (B) were used for analysis at a flow rate of 1 mL/min, with the following gradient: (A/B) 0 min-70/30, 10 min-15/85, 20 min-15/85, 25 min-0/100, 30 min-0/100, 35 min-70/30, 40 min-70/30. Electrospray ionization (ESI) was used in the positive ion mode. In addition, the drying gas flow was set to 12 L/min, the drying gas temperature was set to 350 °C, the capillary voltage was set to 4000 V, and the nebulizer pressure was set to 35 psi. The material was detected at 250 nm using a diode array detector (DAD). Analysis of puerarin palmitate was performed in scan mode (m/z 600–700) and selected ion monitoring (SIM) mode (m/z 655.3).
3
Peptide Molecular Mass Determination
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The molecular mass of each peptide was determined by electrospray ionisation mass spectrometry (ESI–MS) performed on a single quadrupole liquid chromatograph InfinityLab LC/MSD mass spectrometer (InfinityLab LC/MSD, Agilent) coupled to a 1260 Infinity II LC system, Agilent). The reversed-phase HPLC column was a Nucleosil 100-5 C18 Macherey-Nagel (5 μm, 125 mm × 4 mm). The system operated with the standard ESI source and in the positive ionisation mode. For mass spectrometric analysis, the samples were mixed with 50% (v/v) ACN, 50% (v/v) H2O. Peptides were run at a flow rate of 1 ml min−1 with a linear gradient of solvent B over 15 min (A: 99.9% v/v H2O and 0.1% v/v formic acid; B: 99.9% v/v ACN and 0.1% v/v formic acid). Data were acquired, processed and analysed using Agilent OpenLAB CDS (Agilent Technologies) and MestReNova (Mestrelab Research S.L.) software.
4
Bioluminescent Reporter Synthesis and Characterization
5
Analytical Characterization of Compounds
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HPLC analyses were performed on a Merck–Hitachi Elite La Chrom instrument (Tokyo, Japan). Separation conditions were according to the method described by Orfanoudaki et al. [22 (link)], selecting 210 and 330 nm for detection. All samples were membrane filtered (0.45 µm pore size) prior to analysis. NMR experiments were conducted on a Bruker Avance III 400 HD spectrometer (Karlsruhe, Germany) operated at 400.13 MHz (1H-NMR). Mass spectra were recorded on an Agilent InfinityLab LC/MSD system comprising an Agilent 1260 HPLC (Santa Clara, CA, USA) coupled to a single quadrupole MS detector. The experiments were performed in negative ESI mode, using the following settings: capillary voltage 4500 V, drying gas (nitrogen) flow 12 L/min at 320 °C, nebulizer gas (nitrogen) 1.73 bar, and scan range from 50 to 500 m/z.
The purity of isolated compounds was also confirmed by TLC, using silica gel plates from Macherey–Nagel (Düren, Germany) and the solvent system developed by Hartmann et al., i.e., a mixture of n-butanol, acetic acid and water in the ratio 6:2:2 [7 (link)]. Two spray reagents were used for visualization, anisaldehyde/sulphuric acid (universal spray reagent) and ninhydrin (specific for peptides and amino acids). In both cases, the plates were heated to 100 °C for 5 min after spraying and then evaluated in the visible range by naked eye as well as at 366 nm.
The purity of isolated compounds was also confirmed by TLC, using silica gel plates from Macherey–Nagel (Düren, Germany) and the solvent system developed by Hartmann et al., i.e., a mixture of n-butanol, acetic acid and water in the ratio 6:2:2 [7 (link)]. Two spray reagents were used for visualization, anisaldehyde/sulphuric acid (universal spray reagent) and ninhydrin (specific for peptides and amino acids). In both cases, the plates were heated to 100 °C for 5 min after spraying and then evaluated in the visible range by naked eye as well as at 366 nm.
6
HPLC-MS Analysis of Zileuton
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HPLC analysis was performed using an Agilent Technology 1260 infinity hyphenated with mass spectrophotometer (Agilent Technology infinity lab LC/MSD) with an auto sampler system. Chromatographic separation and identification of zileuton was performed using a C18 reverse-phase column (100 mm × 4.6 mm, 5 μm particle size, Discovery C18 from Waters) and a UV detector set at 230 nm using spectral diode array system. The mobile phase consisted of a mixture of ammonium acetate buffer (pH adjusted to 7.5) and 50% methanol at compositions of 10% and 90%, respectively and was freshly prepared every time. The mobile phase flow rate was set at 1 mL/min. The sample injection volume was set to 20 μL and injected into the column using an automatic sample loader.
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