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Lambda dna

Manufactured by Merck Group
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Sourced in Japan, United States

Lambda DNA is a laboratory tool used as a reference standard and control sample in various molecular biology experiments. It is a double-stranded DNA molecule derived from the bacteriophage lambda. Lambda DNA has a well-characterized size and sequence, making it useful for calibrating and verifying the performance of laboratory equipment and procedures.

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Lab products found in correlation

Bovine serum albumin (bsa), by Merck Group (2 mentions) Chitosan, by Merck Group (2 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions) Torula yeast rna, by Merck Group (1 mentions) Cpg dna, by Merck Group (1 mentions) Tri reagent, by Merck Group (1 mentions) Microspin s 400 hr column, by GE Healthcare (1 mentions) Takara ex taq, by Takara Bio (1 mentions) Du 530, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Standard concatenated lambda DNA markers (4 citations) Lambda phage DNA (2 citations)

5 protocols using lambda dna

1

Fluorescence-based Nucleic Acid Quantification

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Example 4

Bacterial Phage Lambda DNA (Sigma-Aldrich), CpG DNA (Enzo Life, Plymouth Meeting, Pa.), Torula yeast RNA (Sigma-Aldrich) were completely dissolved in Tris-acetate-EDTA (TAE) buffer (pH=8) by gentle inversion overnight at 4° C. Lambda DNA, CpG DNA, yeast RNA stock solutions were characterized by spectrophotometry to determine the concentration and purity. Total mouse heart RNA was extracted from C57Bl6 mouse hearts using TRI Reagent (Sigma-Aldrich) and characterized with a NanoDrop spectrophotometer (Thermo Fisher) as described in Feng Y, Chen H, et al., J Biol Chem. 2015; 290: 26688-98.

To measure the fluorescence response in the presence of nucleic acid (NA), serial dilutions of the NA solutions with ratio of 1:3 were prepared in a Corning Costar 96-Well black clear-bottom plate (Thermo Fisher). 50 nM of fluorochrome equivalents, based on the common benzothiazole ring, were added to the NA-containing wells and incubated for 2 hours. Fluorescence was measured with the GloMax-Multi Detection System and snap-in fluorescent optical kits (Promega, Madison, Wis.). EC50 was determined using the dose-response curve fitting function implemented in Prism (GraphPad, La Jolla, Calif.).

US11779661B2. Theranostic nucleic acid binding fluorescent nanoprobes and uses thereof (2023-10-10). The General Hospital Corporation [US]. Inventors: David Sosnovik [US], Lee Josephson [US], Howard Chen [US].
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2

Synthetic Staphylococcal Matrix Assembly

Cited 2 times
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The assembly reaction of the synthetic staphylococcal matrix, starting at pH 7.2, was recorded by measuring the OD600 as a function of time every 30 s for 30 min (35 (link)). Molecular self-assembly reactions were calculated to a final volume of 4 ml, with 0.3% chitosan (medium molecular weight, 75 to 85% deacetylation), 0.15% bovine serum albumin, and 0.015% lambda DNA (all from Sigma) in TSB. The concentration (μM) of tested peptides was calculated for a final volume of 4 ml. Before getting the assembly reaction pH starting points, a calibration record was done using the same reactional tube containing all reagents (auto zero). Acetic acid and NaOH were used to adjust pH, and the reaction temperature was about 30°C. As a negative control, a similarly sized peptide was used, PA-1 (62 (link)).
Gomes Von Borowski R., Chat S., Schneider R., Nonin-Lecomte S., Bouaziz S., Giudice E., Rigon Zimmer A., Baggio Gnoatto S.C., Macedo A.J, & Gillet R. (2021). Capsicumicine, a New Bioinspired Peptide from Red Peppers Prevents Staphylococcal Biofilm In Vitro and In Vivo via a Matrix Anti-Assembly Mechanism of Action. Microbiology Spectrum, 9(2), e00471-21.
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3

Dual-Labeled DNA Probes and PCR Product

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ATTO488- and ATTO647N-labeled 30-bp double-stranded DNA (DNA probe) was custom ordered at Sigma-Aldrich Japan. DNA was prepared by annealing ATTO488-5′-TAC CTT GGG GCC GGT GAG AAT TCG GCC TTT-3′ and ATTO647N-5′-AAA GGC CGA ATT CTC ACC GGC CCC AAG GTA-3′, followed by HPLC purification. Linear 500-bp DNA labeled by ATTO488 and ATTO647N was synthesized by PCR. PCR was performed with TaKaRa Ex Taq (Takara Bio Inc., Shiga, Japan; 5 U μl−1), dNTP Mixture (0.2 mM each), and Lambda-DNA (0.002 μg ml−1) as a template using the 5′-ATTO647N-labeled forward primer and the 5′-ATTO488-labeled reverse primer (0.4 μM each; Sigma-Aldrich Japan). The sequences of primers used were:
Forward primer: ATTO647N-5′-GAT GAG TTC GTG TCC GTA CAA CT-3′
Reverse primer (for 500-bp): ATTO488-5′-GGT TAT CGA AAT CAG CCA CAG CG-3′
PCR products were purified three times using MicroSpin S-400 HR columns (GE Healthcare, Uppsala, Sweden).
Sasaki A., Yamamoto J., Jin T, & Kinjo M. (2015). Raster image cross-correlation analysis for spatiotemporal visualization of intracellular degradation activities against exogenous DNAs. Scientific Reports, 5, 14428.
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4

Synthetic Staphylococcal Matrix Assembly

Cited 2 times
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The assembly reaction of the synthetic staphylococcal matrix, starting at pH 7.2, was recorded by measuring the OD600 as a function of time every 30 s for 30 min (35 (link)). Molecular self-assembly reactions were calculated to a final volume of 4 ml, with 0.3% chitosan (medium molecular weight, 75 to 85% deacetylation), 0.15% bovine serum albumin, and 0.015% lambda DNA (all from Sigma) in TSB. The concentration (μM) of tested peptides was calculated for a final volume of 4 ml. Before getting the assembly reaction pH starting points, a calibration record was done using the same reactional tube containing all reagents (auto zero). Acetic acid and NaOH were used to adjust pH, and the reaction temperature was about 30°C. As a negative control, a similarly sized peptide was used, PA-1 (62 (link)).
Gomes Von Borowski R., Chat S., Schneider R., Nonin-Lecomte S., Bouaziz S., Giudice E., Rigon Zimmer A., Baggio Gnoatto S.C., Macedo A.J, & Gillet R. (2021). Capsicumicine, a New Bioinspired Peptide from Red Peppers Prevents Staphylococcal Biofilm In Vitro and In Vivo via a Matrix Anti-Assembly Mechanism of Action. Microbiology Spectrum, 9(2), e00471-21.
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5

Genomic DNA Extraction from Boerhavia Leaves

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The molecular study was conducted at the Biotechnology laboratory, National Horticultural Research Institute (NIHORT), Ibadan, Nigeria. Fresh and young leaves of the three Boerhavia species studied were collected for genomic DNA extraction using modified Dellaporta procedure (Dellaporta et al. 1983) . The quality and concentration of DNA was assessed by gel electrophoresis using 2% agarose with known concentrations of undigested lambda DNA (Sigma, St. Louis, MO, USA). Quantification of DNA was done using absorbance measurement in a spectrophotometer (Beckman Coulter DU530) at 260 nm.
Azeez S.O., Matthew J.O., Olaoluwa E., Adegboyega G., & Akinyoola I. (2020). Morphological and molecular studies of three species of Boerhavia L. from Ile-Ife, Nigeria. Deleted Journal, 22(2), 225-236.
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