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Ac 55ts

Manufactured by Olympus

The AC-55TS is a laboratory equipment product designed for general use in research and testing environments. It serves as a tool for performing specific functions within the laboratory setting. The core function of the AC-55TS is to provide a controlled and reliable means of conducting various experiments and analyses.

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2 protocols using ac 55ts

1

In situ AFM Measurements of Ionic Liquid Gating

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The in situ AFM measurements were carried out in a Cypher (Asylum Research)
atomic force microscope. An integrated blueDrive (Asylum Research)
laser was used to actuate the oscillation of the cantilever (AC-55
TS, Olympus) in the IL. During AFM scanning, the cantilever, gate
electrode plate (Au), and SCO sample were all covered by the IL (Figure 1a), and the gate
voltage was applied by an external source meter (Keithley 2400). The
ionic liquid 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide
(EMIM-TFSI), was used for all IL gating experiments. The IL and the
devices were separately baked at 380 K in high vacuum (10–7 mbar) for at least 12 h before the gating experiments were carried
out. Except for the H2O-vol % dependent XPS measurements,
dried IL was otherwise used for all measurements.
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2

Atomic Force Microscopy of Titin Molecules

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Titin samples were imaged with a high-resolution atomic force microscope (Cypher, AsylumResearch, Santa Barbara, CA). Dehydrated samples were imaged in tapping mode with a stiff cantilever (Olympus AC160 or AC55TS with nominal tip radii of 9 and 7 nm, respectively) at a line-scan rate of 3–6 Hz and pixel resolution of 0.5 - 2 nm. Hydrated samples were scanned in tapping mode with a soft, high-resonance-frequency cantilever (Olympus BL-RC150VB, BioLever B, nominal tip radius 25 nm) a a line-scan rate of 2–3 Hz and a pixel resolution of 2–4 nm. Images were corrected for flatness of field and color contrast (offset and range) by using built-in algorithms of the AFM driver software. Topographical distance (e.g., filament width) measurements were corrected for tip convolution as published earlier [29] (link). Accordingly, titin molecule width (W) was calculated as where Wm is measured width at half of the maximum, r is tip radius and h is topographical height in the filament axis. The width of topographical gaps (G) was obtained as where Gm is the measured gap width.
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