Anti stat6 sc 621

All cells and tissues were processed and analyzed as previously described^{5 (link)}. For RT-PCR, total RNA was extracted using a TRIzol extraction method. The sequences of primers for RT-PCR and ChIP assays are listed in Supplementary Tables S2 and S3^{5 (link)}. Flag-tagged MafB was purified with M2 Affinity Gel as previously described^{5 (link)}, and Myc/DDK-tagged LXRα was obtained from OriGene. Immunoblot and ChIP analyses used the following antibodies: anti-β-actin (ab8226; Abcam), anti-GAPDH (sc-25778; Santa Cruz), anti-MafB (MAB3810; R&D Systems), anti-Stat6 (sc-621; Santa Cruz), anti-pStat6 (sc-11762; Santa Cruz), anti-LXRα (ab41902; abcam), anti-Flag (M2) or anti-HA Affinity Gel (Sigma), goat anti-rabbit IgG-HRP (sc-2054; Santa Cruz), IRDye 800CW–conjugated goat anti–mouse and anti–rabbit IgG (926-32210 and 926-32211, respectively; LI-COR), and IRDye 800–conjugated anti-Flag and anti-HA (600-432-383 and 600-432-384, respectively; Rockland). Immunocomplexes were visualized with an Odyssey Infrared Imaging System (LI-COR).

All commercial antibodies and reagents were purchased from the following sources: anti-Foxp3 (ab22510, 1:200 (IHC, IF), 1:1000 (WB) dilution) antibody was from Abcam; anti-actin (A5316, 1:5,000 dilution), anti-Flag (F7425, 1:5000 dilution) antibody was from Sigma-Aldrich; anti-Iba-1 (019–19741, 1:400 dilution) antibody was from Wako; anti-CD200R (AF2554, 1:500 dilution) antibody was from R&D Systems; Hoechst (33342, 1:1000 dilution) antibody was from Invitrogen; anti-Arg1 (sc-20150, 1:1000, dilution), anti-phopho-STAT6 (sc-11762, 1:1000 dilution), anti-STAT6 (sc-621, 1:1000 dilution) antibodies were from Santa Cruz Biotechnology; CD200 recombinant protein (2554-CD, 500 ng/ml) were from R&D Systems; and bacterial LPS were from Sigma-Aldrich.