KELLY cells were purchased from Sigma (St. Louis, MO) and cultured in DMEM:F12, supplemented with 10% FBS. HEK293TN cells were cultured in DMEM with 10% FBS. Transient transfection was performed by using jetPRIME (Polyplus transfection, #114-07) per the manufacturer’s instructions. CoIP analysis was performed essentially as previously described[21 (link)]. Briefly, two days post-transfection, whole cell lysates of HEK293TN cells were prepared and used for V5-IP (Invitrogen, #R960-25). In the case of KELLY cells, nuclear extracts were isolated and subjected to immunoprecipitation with anti-MYCN antibody (Santa Cruz, #sc-53993), followed by immunoblotting with anti-EYA1 antibody (Prosci, #25-067). The following antibodies were used in western blot: α-Flag (Thermo Fisher Scientific, #MA1-91878) and β-actin (Sigma, #A5316).
V5 ip
Manufactured by Thermo Fisher Scientific
The V5-IP is a versatile laboratory equipment designed for a variety of applications. It serves as a compact and reliable tool for researchers and scientists. The core function of the V5-IP is to provide a controlled environment for various laboratory processes. However, a more detailed description while maintaining an unbiased and factual approach is not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Anti mycn antibody, by Santa Cruz Biotechnology (2 mentions)
α flag, by Thermo Fisher Scientific (2 mentions)
Jetprime, by Polyplus Transfection (2 mentions)
β actin, by Merck Group (2 mentions)
Kelly cells, by Santa Cruz Biotechnology (2 mentions)
Anti eya1 antibody, by ProSci (2 mentions)
Kelly cells, by Merck Group (2 mentions)
2 protocols using v5 ip
1
Coimmunoprecipitation of MYCN and EYA1 in KELLY cells
2
Coimmunoprecipitation of MYCN and EYA1 in KELLY cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
KELLY cells were purchased from Sigma (St. Louis, MO) and cultured in DMEM:F12, supplemented with 10% FBS. HEK293TN cells were cultured in DMEM with 10% FBS. Transient transfection was performed by using jetPRIME (Polyplus transfection, #114-07) per the manufacturer’s instructions. CoIP analysis was performed essentially as previously described[21 (link)]. Briefly, two days post-transfection, whole cell lysates of HEK293TN cells were prepared and used for V5-IP (Invitrogen, #R960-25). In the case of KELLY cells, nuclear extracts were isolated and subjected to immunoprecipitation with anti-MYCN antibody (Santa Cruz, #sc-53993), followed by immunoblotting with anti-EYA1 antibody (Prosci, #25-067). The following antibodies were used in western blot: α-Flag (Thermo Fisher Scientific, #MA1-91878) and β-actin (Sigma, #A5316).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!