Anti il 17a apc tc11 18h10.1

Manufactured by BioLegend

Sourced in United States

Anti-IL-17A-APC (TC11-18H10.1) is a flow cytometry reagent that binds to interleukin-17A (IL-17A). It is conjugated with the fluorescent dye Allophycocyanin (APC).

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Lab products found in correlation

Foxp3 pe fjk 16s, by Thermo Fisher Scientific (1 mentions) Live dead fixable aqua dead cell stain, by Thermo Fisher Scientific (1 mentions) Anti cd4 pe l3t4 gk1.5, by BD (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions)

Spelling variants of this product

IL-17A (121 citations) Anti-IL-17A (34 citations) IL-17A (TC11-18H10.1) (16 citations) TC11-18H10.1 (16 citations) Anti-IL-17A (TC11-18H10.1) (12 citations) Anti-IL-17A APC (8 citations) IL-17A (TC11-18H10, (3 citations) Anti-IL-1ß (2 citations)

2 protocols using anti il 17a apc tc11 18h10.1

Multiparameter Immunophenotyping of Lymphocytes

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Single-cell leukocyte suspensions from the spleen and draining lymph nodes were generated by gentle dissection. The following mAbs were used for staining: anti CD4-PE (L3T4 GK1.5), and anti-B220-Fluorescein isothiocyanate (FITC; RA3-6B2) from BD Biosciences; anti CD8a-Pacific Blue (53–6.7) from eBioscience (Waltham, MA, USA); anti-CD1d-FITC (1B1), anti-CD5-PE (53–7.3), anti-CD11b-PerCP/Cy5.5 (M1/70), anti-CD19-APC (6D5), anti-CD19-PE-Cy7 (6D5), anti-CD90.2-PE/Cy7 (30-H12), anti-F4/80-APC/Cy7 (BM8), anti-IL-10-PE (JES5-16E3), anti-IL-10-APC (JES5-16E3), and anti-IL-17A-APC (TC11-18H10.1) from BioLegend; and FoxP3-PE (FJK-16 s) from Invitrogen (Carlsbad, CA, USA). LIVE/DEAD Fixable Aqua Dead Cell Stain (Invitrogen) was used to detect dead cells. Single-cell suspensions (10⁶ cells) from the spleen and draining lymph nodes were stained at 4 °C for 20 min using mAbs at predetermined optimal concentrations.

Mizumaki K., Horii M., Kano M., Komuro A, & Matsushita T. (2021). Suppression of IL-23-mediated psoriasis-like inflammation by regulatory B cells. Scientific Reports, 11, 2106.

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Single Cell Immunophenotyping by Flow Cytometry

Cited 1 time

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Single cell suspensions for flow cytometry were prepared as described (23 (link)) and stained with the following antibodies: anti-CD45-FITC (30-F11), anti-CD3-BV421 (17A2), anti-CD4-PE (A161A1), anti-IFNγ-Alexa700 (XMG1.2), anti-IL-4-APC (11B11), and anti-IL-17A-APC (TC11–18H10.1) (all from Biolegend). Data were acquired with a BD LSRII flow cytometer (BD Biosciences, San Diego, CA) and analyzed with FlowJo.

Lewis B.W., Amici S.A., Kim H.Y., Shalosky E.M., Khan A.Q., Walum J., Gowdy K.M., Englert J.A., Porter N.A., Grayson M.H., Britt RD J.r, & Guerau-de-Arellano M. (2022). PRMT5 in T cells drives Th17 responses, mixed granulocytic inflammation and severe allergic airway inflammation. Journal of immunology (Baltimore, Md. : 1950), 208(7), 1525-1533.

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