Human il 6 quantikine kit

Manufactured by R&D Systems

Sourced in United States

The Human IL-6 Quantikine Kit is a solid-phase enzyme-linked immunosorbent assay (ELISA) designed to measure human interleukin 6 (IL-6) levels in cell culture supernates, serum, and plasma. The kit uses a quantitative sandwich enzyme immunoassay technique to determine the concentration of IL-6 in the sample.

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Lab products found in correlation

Multimode microplate reader, by Tecan (1 mentions)

Close spelling variants of this product

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3 protocols using human il 6 quantikine kit

Plasma Cytokine Profiling Protocol

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Plasma samples were analyzed for IL-6, IL-18, fractalkine and TRAIL by following specific commercial plate-based ELISAs: (1) Human IL-6 Quantikine Kit (R&D Systems, USA), (2) Human IL-18 Kit (IBL, Japan), (3) Human Fractalkine Quantikine Kit (R&D Systems, USA), and (4) Human TRAIL Quantikine Kit (R&D Systems, USA). Assays were performed according to the manufacturer's instructions.

Wu Y.Y., Hsu J.L., Wang H.C., Wu S.J., Hong C.J, & Cheng I.H. (2015). Alterations of the Neuroinflammatory Markers IL-6 and TRAIL in Alzheimer's Disease. Dementia and Geriatric Cognitive Disorders EXTRA, 5(3), 424-434.

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Quantification of IL-6 Secretion

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To analyze IL-6 secretion, cells were treated with 0.0, 0.5, 1, or 2 μM of CDDP for 72 h. Then, the supernatants were collected and ELISA was performed using the human IL-6 Quantikine kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol. Recombinant human IL-6 was used for calibration. The absorbance at 450/570 nm was measured with a multimode microplate reader (Tecan, San Jose, CA, USA).

Wang X., Li Y., Dai Y., Liu Q., Ning S., Liu J., Shen Z., Zhu D., Jiang F., Zhang J, & Li Z. (2016). Sulforaphane improves chemotherapy efficacy by targeting cancer stem cell-like properties via the miR-124/IL-6R/STAT3 axis. Scientific Reports, 6, 36796.

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Quantifying IL-6 Levels via ELISA

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IL-6 levels were assessed by enzyme-linked immunosorbent assays using the Human IL-6 Quantikine kit (R&D Systems Inc., Minneapolis, MN). The sensitivity of the test was < 0.70 pg/mL, and the inter-assay and intra-assay coefficients were < 10%.

Kacerovsky M., Romero R., Pliskova L., Bolehovska R., Hornychova H., Matejkova A., Vosmikova H., Andrys C., Kolackova M., Laudański P., Pelantova V., Jacobsson B, & Musilova I. (2019). Presence of Chlamydia trachomatis DNA in the amniotic fluid in women with preterm prelabor rupture of membranes. The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians, 34(10), 1586-1597.

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