The cytotoxicity of the compound was detected using the WST-1 cell proliferation and cytotoxicity assay kit (Catalog NO: 05015944001, Roche, USA). Vero (2×104 per well), 293T and RD cells (3×104 per well in 100 µl 10% FBS-DMEM medium) were cultured in 96-well plates at 37 °C under 5% CO2, followed by the addition of 50 µl of the compound solution with concentrations ranging from 0.39-100 µM. The cells were incubated at 37°C for 24 h, and then 10 µl of the WST-1 reagent was added to each well. The signals were read at 490 nm with a microplate reader (Bio-Rad, USA). The percentage of viable cells after treatment with the various concentrations of compound was calculated as follows: %inhibition = 100 × (OD490) treated sample / (OD490) cell control sample.
Wst 1 cell proliferation and cytotoxicity assay kit
Manufactured by Roche
Sourced in United States, Germany
The WST-1 cell proliferation and cytotoxicity assay kit is a colorimetric assay for the quantification of cell viability and proliferation. The kit utilizes a tetrazolium salt which is reduced by metabolically active cells, resulting in the formation of a colored formazan dye that can be measured spectrophotometrically.
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3 protocols using wst 1 cell proliferation and cytotoxicity assay kit
1
Cytotoxicity Evaluation of Compounds
2
Cell Viability Assay in 96-well Plates
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The viability of cells cultured in 96-well plates was then measured using the WST-1 cell proliferation and cytotoxicity assay kit (Roche, Mannheim, Germany) and according to the manufacturer’s instructions. Absorbance at the experimental wavelength of 440 nm and the reference wavelength of ~630 nm were monitored using Spectra Max M5 (Molecular Devices, Sunnyvale, CA, USA).
3
Quantifying Cell Viability with WST-1 Assay
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Cell viability was determined with a WST-1 cell proliferation and cytotoxicity assay kit (Roche, Indianapolis, IN). The cells were seeded in 96-well plates with 100 μL of culture medium, then transfected with miRNA mimic and miRNA inhibitor. The medium was replenished at specific time points, and 10 μL of WST-1 solution added before incubation for 1 h at 37°C. Subsequently, the absorbance was measured at 450 nm.
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