Af488 goat anti mouse

Manufactured by Jackson ImmunoResearch

AF488 Goat Anti-Mouse is a fluorophore-conjugated secondary antibody used for the detection and visualization of mouse primary antibodies in various immunoassays and microscopy applications.

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Lab products found in correlation

Af647 donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) Donkey anti rabbit af594, by Jackson ImmunoResearch (1 mentions) Mouse anti ctbp2, by BD (1 mentions) Mouse anti gria2, by Merck Group (1 mentions) Donkey anti mouse af488, by Jackson ImmunoResearch (1 mentions) Donkey anti goat af647, by Jackson ImmunoResearch (1 mentions) Flojo software, by BD (1 mentions) Mopc 21, by BD (1 mentions)

Spelling variants of this product

AF488-conjugated goat anti-mouse antibody (2 citations) Goat anti-mouse AF488-IgG (2 citations)

2 protocols using af488 goat anti mouse

Immunofluorescence Antibody Protocol

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The following primary antibodies were used: mouse IgG1 anti-SIRT3 antibody (1:200; Novus Biologicals; RRID:AB_2818991); goat anti-Oncomodulin antibody (OCM; 1:1000; Santa Cruz; RRID:AB_2267583), rabbit anti-Myosin7a (MYO7; 1:200; Proteus; RRID:AB_10013626) mouse anti-CTBP2 (aka C-Terminal Binding Protein 2; 1:200; BD Transduction Laboratories; RRID:AB_399431), and mouse anti-GRIA2 (aka GluR2/GluA2; 1:2000; Millipore; RRID:AB_2113875). The following secondary antibodies were purchased from Jackson Immuno Research: Donkey Anti-Mouse AF488 (1:500; RRID:AB_2340849), Donkey Anti-Rabbit AF594 (1:500; RRID:AB_2340622), Donkey Anti-Rabbit AF647 (1:200; RRID:AB_2340625), Donkey Anti-Goat AF647 (1:200; RRID:AB_2340438), Goat Anti-Mouse AF594 (IgG1, 1:500; RRID:AB_2338885), AF488 Goat Anti-Mouse (IgG2a, 1:500; RRID:AB_2338855). For the images in Fig 1, an AF568 Goat Anti-Mouse (IgG1, 1:200, Thermo Fisher, RRID: AB_2535766) was used.

Patel S., Shah L., Dang N., Tan X., Almudevar A, & White P.M. (2020). SIRT3 promotes auditory function in young adult FVB/nJ mice but is dispensable for hearing recovery after noise exposure. PLoS ONE, 15(7), e0235491.

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Immunofluorescence Staining of Ea.hy926 Cells

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Ea.hy926 cells were harvested with PBS containing 1 mM EDTA and incubated with primary antibody for 1 hour followed by secondary antibody for 30 minutes, with washes performed in between. Isotype control antibody MOPC-21 was purchased from BD Biosciences (San Jose, CA). Secondary antibody AF488 Goat anti-Mouse was purchased from Jackson ImmunoResearch (Westgrove, PN). Data was analyzed using Flojo Software (Becton Dickinson, Franklin Lakes, NJ).

Peterson J.A., Maroney S.A., Martinez N.D, & Mast A.E. (2021). A major reservoir for heparin-releasable TFPIα is extracellular matrix. Arteriosclerosis, thrombosis, and vascular biology, 41(6), 1942-1955.

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