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7 amimo actinomycin

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7-Amino-Actinomycin is a fluorescent dye used in laboratory research applications. It binds to DNA and can be used to stain and detect nucleic acids.

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Lab products found in correlation

Annexin 5 pe, by BD (2 mentions) Cflow software, by BD (2 mentions) Accuri c6 flow cytometer, by BD (1 mentions) Accuri c6, by BD (1 mentions)

2 protocols using 7 amimo actinomycin

1

Macrophage Apoptosis Assay with MCL

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Mouse primary peritoneal macrophages were treated with indicated concentrations of MCL for 18 h in the presence or absence of PGN (25 mg/L), and then were harvested and labeled with PE-Annexin V and 7-Amimo-Actinomycin (7-AAD) provided by BD Pharmingen (San Diego, USA), following the manufacturer’s instructions. Samples were examined by a flow cytometer BD AccuriTM C6 (BD, San Jose, USA). Data were analyzed using CFlow software (BD, San Jose, USA).
Jiang X., Wang Y., Qin Y., He W., Benlahrech A., Zhang Q., Jiang X., Lu Z., Ji G, & Zheng Y. (2017). Micheliolide provides protection of mice against Staphylococcus aureus and MRSA infection by down-regulating inflammatory response. Scientific Reports, 7, 41964.
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2

Apoptosis Induction Assay in Raw264.7

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Raw264.7 was treated with indicated concentrations of MCL for 18 h with or without LPS (100 ng/mL), and then was harvested and labeled with PE-Annexin V and 7-Amimo-Actinomycin (7-AAD) provided by BD Pharmingen (San Diego, USA), following manufacturer’s instructions. Samples were examined by a flow cytometer BD AccuriTM C6 (BD, San Jose, USA). Data were analyzed using CFlow software (BD, San Jose, USA).
Qin X., Jiang X., Jiang X., Wang Y., Miao Z., He W., Yang G., Lv Z., Yu Y, & Zheng Y. (2016). Micheliolide inhibits LPS-induced inflammatory response and protects mice from LPS challenge. Scientific Reports, 6, 23240.
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