E501 module analyzer

A fasting blood test was collected from all included patients from the right antecubital vein. The evaluated parameters and biomarkers included lymphocytes (reference value (RV): cell count × 10⁹/L), hemoglobin (RV: 12–16 g/dL), platelets (RV: 150–400 count × 10⁹/L), lactate dehydrogenase (LDH) level (135–250 U/L), serum creatinine (RV: 0.5–1.1 mg/dL), aspartate aminotransferase (AST) (RV < 32 U/L), (RV < 40 U L); D-dimer (RV < 500 ng/dL), serum ferritin (RV: 15–150 ng/mL), interleukin-6 (RV: <5.9 pg/mL), high-sensitivity C-reactive protein (hs-CRP) (RV 1–5 mg/L), NT-ProBNP (0–125 pg/mL), and high-sensitivity Troponin T (hs-TnT) (RV: <14 pg/mL).
All tests were performed in a certified clinical laboratory (ISO 9001:2015). Inflammatory markers were evaluated using a particle-enhanced immunoturbidimetric, colorimetric (e501 Module Analyzer^®, Roche Diagnostics, Basel, Switzerland) immunoassay system on IMMULITE^® 2000 for interleukin-6 (IMMULITE^® 2000 IL-6, Siemens Healthcare Diagnostic, Marburg, Germany). Cardiac biomarkers were measured by electrochemiluminescence immunoassay with a cobas^® 6000 c 601 analyzer (Roche Diagnostics, Basel, Switzerland). D-dimer results were obtained by a turbidimetric test using an ACL TOP 500^® hemostasis testing system (Werfen Company, Cuenca, Spain).

Procalcitonin measurement in plasma was performed by electrochemiluminescence immunoassay on a chemistry analyser (Cobas 6000, Roche Diagnostics, Meylan, France) with a limit of detection of 0.02 ng/mL. Serum C-reactive protein was measured by particle enhanced immunoturbidimetric assay (e501 Module Analyzer, Roche Diagnostics) with a limit of detection of 0.15 mg/dL. The determinations were made on admission to the post-surgical critical care unit and during their stay at 8, 16, 24 and 72 h.

Procalcitonin (PCT) measurement in plasma was performed by electrochemiluminescence immunoassay on a chemistry analyzer (Cobas 6000, Roche Diagnostics, Meylan, France) in samples collected in the first 12 hours following diagnosis of sepsis or in the first 12 hours following surgery in the case of the surgical controls; limit of detection 0.02 ng/mL. Serum C-reactive protein (CRP) was measured by particle enhanced immunoturbidimetric assay (e501 Module Analyzer, Roche Diagnostics); limit of detection 0.15 mg/dL.
Both samples for HLA-DRA and PCT quantification were stored at −80 °C to be profiled in the same moment with the same platforms to avoid bias due to multiplatform testing.