Plasma glucose was measured using the hexokinase method (Quailigentglu, Sekisui, Japan), and HbA1c was measured via high-performance liquid chromatography (G8 Elution Buffer, Tosoh, Tokyo, Japan). The plasma insulin concentration was determined via electrochemiluminescenceimmunoassay (ElecsysInsulin, Roche Diagnostics GmbH, Mannheim, Germany). Insulin resistance (homeostatic model assessment for insulin resistance [HOMA-IR]) and secretion (HOMA-β) were calculated by homeostasis model assessment27 (link).
G8 elution buffer
Manufactured by Tosoh
Sourced in Japan
The G8 Elution Buffer is a laboratory reagent designed to facilitate the elution process in various analytical techniques. It serves as a buffer solution to help release and recover target analytes from a solid phase or matrix. The core function of the G8 Elution Buffer is to provide the necessary chemical environment to enable the efficient separation and collection of the desired substances.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using g8 elution buffer
1
Measuring Metabolic Parameters
2
Measuring Insulin Resistance and Secretion
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Plasma glucose was measured using the hexokinase method (Quailigentglu, Sekisui, Japan), and HbA1c was measured via high-performance liquid chromatography (G8 Elution Buffer, Tosoh, Tokyo, Japan). The plasma insulin concentration was determined via electrochemiluminescence immunoassay (ElecsysInsulin, Roche Diagnostics GmbH, Mannheim, Germany). Insulin resistance (homeostatic model assessment for insulin resistance [HOMA-IR]) and secretion (HOMA-β) were calculated by homeostasis model assessment49 (link).
3
Glycemic Biomarker Measurement Methods
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We measured plasma glucose using the hexokinase method (Quailigentglu, Sekisui, Japan), glycated albumin using a glycol-albumin assay kit (LUCICARGA-L; Asahi Kasei Pharma Co., Tokyo, Japan), and HbA1c via high-performance liquid chromatography (G8 Elution Buffer; Tosoh, Tokyo, Japan).
4
Glucose, HbA1c, and Insulin Assays
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Plasma glucose was measured using the hexokinase method (Quailigentglu, Sekisui, Japan), and HbA1c was measured via high-performance liquid chromatography (G8 Elution Buffer, Tosoh, Tokyo, Japan). The plasma insulin concentration was determined via electrochemiluminescence immunoassay (Elecsys Insulin, Roche Diagnostics GmbH, Mannheim, Germany). Insulin resistance (homeostatic model assessment for insulin resistance [HOMA-IR]) and secretion (HOMA-β) were calculated by homeostasis model assessment [30 (link)].
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