Imager2 fluorescent microscope

Manufactured by Zeiss

Sourced in Germany

The Imager2 is a fluorescent microscope designed for high-resolution imaging. It features advanced optics and detection systems to capture detailed fluorescent signals from samples. The Imager2 is capable of providing clear, high-quality images for a variety of applications.

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Fluorescent microscope (298 citations)

2 protocols using imager2 fluorescent microscope

Avian Chromosomal Karyotyping and Comparative Genomics

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Conventionally stained metaphases (Giemsa 5% in 0.07 M phosphate buffer, pH 6.8) were used for diploid number definition and karyotype ordering. We analyzed slides using the 100x immersion objective of a Leica DM1000 microscope. For each individual, 30 conventionally stained metaphases were captured and analyzed using the GenAsis software.
FISH experiments were conducted following standard protocols as described by de Oliveira et al., 2010 [22 (link)]. Whole chromosome probes from chicken (GGA), corresponding to pairs 1 to 10 and Leucopternis albicollis (LAL) homologous to GGA1 (LAL 3, 6, 7, 15 and 18), 2 (LAL 2, 4, and 20), 3 (LAL 9, 13, 17 and 26), 4 (LAL 1 and 16), 5 (LAL 5), 6 (LAL 3), 7 (LAL 8), 8 (LAL 10), 9 (LAL 12) and 10 (LAL 19) [22 (link)], were obtained by flow sorting, and labeled by DOP-PCR with biotin (detected with avidin-CY3) or fluorescein. Chromosomes were counterstained with DAPI. Images were recorded using a 63x immersion objective of a Zeiss Imager2 fluorescent microscope and analyzed with Axionvision 4.8 software (Zeiss, Germany). Comparisons were based on the avian putative ancestral karyotype (PAK), in which pairs PAK 1–11 corresponded to GGA1-GGA3, GGA4q, GGA5-GGA9, GGA4p and GGA10, respectively [23 (link)].

Furo I.D., Monte A.A., dos Santos M.D., Tagliarini M.M., O´Brien P.C., Ferguson-Smith M.A, & de Oliveira E.H. (2015). Cytotaxonomy of Eurypyga helias (Gruiformes, Eurypygidae): First Karyotypic Description and Phylogenetic Proximity with Rynochetidae. PLoS ONE, 10(12), e0143982.

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Tissue Collection and Immunohistochemistry in Mice

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For tissue collection for histology, mice were anesthetized and transcardially perfused with PBS and 4% paraformaldehyde (PFA). Brains were dissected and fixed in 4% PFA overnight, then placed in 30% sucrose for 2 days, and then embedded in Tissue-Tek O.C.T Compound. Cryosections (10 μm) were cut and stored at -80 °C. Cryosections were blocked in PBS containing 10% donkey serum in 0.1% Triton X-100 solution for 2 h and incubated overnight at 4°C with primary antibodies. Next, sections were washed in PBS-T (Tween-20, 0.05%) and incubated with corresponding secondary antibodies for 2 h at room temperature. Specimens were mounted with a mounting medium containing DAPI. Images were obtained on a Zeiss Axio Imager 2 fluorescent microscope. All antibodies were diluted in PBS solution containing 3% of donkey serum. For reagent specifications, catalog numbers, and concentrations, see key resources table.

Ochocka N., Segit P., Wojnicki K., Cyranowski S., Swatler J., Jacek K., Grajkowska W, & Kaminska B. (2023). Specialized functions and sexual dimorphism explain the functional diversity of the myeloid populations during glioma progression. Cell reports, 42(1).

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