For western blot assay, HCC cell lines after transfection or without processing were harvested in lysis buffer. The concentration of total protein was evaluated by BCA protein assay kit (Thermo Fisher Scientific, lnc.). Then the protein was separated by 12% SDS-PAGE and transferred onto PVDF membranes (Thermo Fisher Scientific, lnc.). After incubation with 5% milk at room temperature for 2 h, the membranes were maintained with primary antibody as indicated at 4 ℃ overnight and then incubated with secondary antibodies at room temperature for 2 h. Protein bands were visualized with an enhanced chemiluminescence (ECL) assay. (Millipore, Billerica, MA). GAPDH was acted as endogenous control. The primary antibodies used as follow: anti-KLF7 (398576, Santa Cruz), anti-GAPDH (60004–1-Ig, Proteintech), anti-VPS35 (157220, Abcam), anti-CCDC85C (TA330857, ORIGENE), anti-active β-catenin (19807, Cell Signaling Technology), anti-β-actin (47778, Santa Cruz).
Anti ccdc85c
Manufactured by OriGene
Anti-CCDC85C is a laboratory reagent used for the detection and analysis of the CCDC85C protein. CCDC85C is a coiled-coil domain containing protein that is involved in various cellular processes. The anti-CCDC85C reagent can be used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to study the expression and localization of the CCDC85C protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti vps35, by Abcam (2 mentions)
Anti β actin, by Santa Cruz Biotechnology (2 mentions)
Anti klf7, by Santa Cruz Biotechnology (2 mentions)
Anti active β catenin, by Cell Signaling Technology (2 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
Anti gapdh 60004 1 ig, by Proteintech (1 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti ccdc85c
1
Western Blot Analysis of HCC Cell Lines
2
Western Blot Assay for HCC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For western blot assay, HCC cell lines after transfection or without processing were harvested in lysis buffer. The concentration of total protein was evaluated by BCA protein assay kit (Thermo Fisher Scienti c, lnc.). Then the protein was separated by 12% SDS-PAGE and transferred onto PVDF membranes (Thermo Fisher Scienti c, lnc.). After incubation with 5% milk at room temperature for 2 h, the membranes were maintained with primary antibody as indicated at 4 ℃ overnight and then incubated with secondary antibodies at room temperature for 2 h. Protein bands were visualized with an enhanced chemiluminescence (ECL) assay. (Millipore, Billerica, MA). GAPDH was acted as endogenous control. The primary antibodies used as follow: anti-KLF7 (398576, Santa Cruz), anti-GAPDH (60004-1-Ig, Proteintech), anti-VPS35 (157220, Abcam), anti-CCDC85C (TA330857, ORIGENE), anti-active β-catenin (19807, Cell Signaling Technology), anti-β-actin (47778, Santa Cruz).
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