Donor hearts were obtained from organ procurement organizations under an IRB-approved protocol from Cedars-Sinai Medical Center and processed as described by RR Makkar et al.7 (link) with modifications. These samples were obtained following informed consent of the donor or next of kin. All methods were carried out in accordance with relevant guidelines and regulations. A combination of atrial and septal tissue was used to seed explant fragments without previous collagenase digestion. Explants were seeded onto CellBIND surface culture flasks (Corning) for 10–21 days before harvest of explant-derived cells (EDC) and formation of cardiopsheres in ultra-low attachment surface flasks (Corning) for 3 days. CDCs were obtained by seeding cardiospheres onto fibronectin-coated dishes and cultured until passage 5. All cultures were maintained at 5% CO2, 5% O2 at 37 °C, using IMDM (GIBCO; supplemented with 20% bovine serum (Equafetal, Atlas), 0.5 μg/mL gentamycin, and 99 μM 2-mercaptoethanol).
Cellbind surface culture flasks
Manufactured by Corning
CellBIND surface culture flasks are specialized cell culture vessels designed to provide a reliable and consistent surface for cell attachment and growth. The CellBIND surface is a proprietary treatment that enhances the wettability and hydrophilicity of the flask, promoting improved cell adhesion and proliferation. The flasks are available in a range of sizes and formats to accommodate various cell culture needs.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cellbind surface culture flasks
1
Cardiosphere-Derived Cell Isolation Protocol
2
Cardiosphere-Derived Cell Isolation Protocol
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Cardiosphere-derive cells (CDCs). Donor hearts were obtained from organ procurement organizations under an IRB-approved protocol and processed as described by RR Makkar et al. (20) with modifications. A combination of atrial and septal tissue was used to seed explant fragments without previous collagenase digestion. Explants were seeded onto CellBIND surface culture flasks (Corning) for 10-21 days before harvest of explant-derived cells (EDC) and formation of cardiopsheres in ultra-low attachment surface flasks (Corning) for 3 days. CDCs were obtained by seeding cardiospheres onto fibronectin-coated dishes and cultured until passage 5. All cultures were maintained at 5% CO2, 5% O2 at 37°C, using IMDM (GIBCO; supplemented with 20% bovine serum (Equafetal, Atlas), 0.5 μg/mL gentamycin, and 99 μM 2-mercaptoethanol).
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