Lysotracker dnd 99 l 7528

Manufactured by Thermo Fisher Scientific

Lysotracker DND-99 (L-7528) is a fluorescent dye used to label and track acidic organelles, such as lysosomes, in live cells. It is a membrane-permeant probe that accumulates in acidic compartments. The dye exhibits a fluorescence signal that increases upon binding to acidic environments.

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Lab products found in correlation

Ym201636, by Abcam (1 mentions) Apilimod, by US Biological (1 mentions) Dq green bsa, by Thermo Fisher Scientific (1 mentions) Human osteoactivin gpnmb anti goat, by R&D Systems (1 mentions) Mouse anti lamp1, by Santa Cruz Biotechnology (1 mentions) Fetal bovine serum (fbs), by Euroclone (1 mentions) Dulbecco modified eagle medium (dmem), by Euroclone (1 mentions)

2 protocols using lysotracker dnd 99 l 7528

Lysosomal Marker Antibody Staining

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Mouse monoclonal anti-LampI (clone H4A3) antibody (sc-2001) was obtained from Santa Cruz Biotechnology. Mouse monoclonal anti-maltose-binding protein (MBP) antibody (E8032) and anti-mouse Alexa 555 secondary antibody (4409S) were purchased from New England Biolabs. Lysotracker DND-99 (L-7528) was purchased from Thermo Fisher. YM201636 was purchased from Abcam (ab141370), Apilimod from US Biological (002800).

Guscott B., Balklava Z., Safrany S.T, & Wassmer T. (2016). A cell-permeable tool for analysing APP intracellular domain function and manipulation of PIKfyve activity. Bioscience Reports, 36(2), e00319.

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Quantifying Lysosomal Activity in Cells

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Reagents were obtained from the following sources: human TFEB (4240) anti‐rabbit from Cell Signaling Technology; antibody to murine TFEB from Bethyl laboratories (A303‐673A); human TFE3 anti‐rabbit from Sigma Aldrich (#SAB4200803), antibody to Cadherin 16 from Novus Biologicals (NBP1‐59248); human osteoactivin/GPNMB antigoat from R&D Systems; mouse anti‐LAMP1 was from Santa Cruz (1D4B). DMEM, RPMI, and fetal bovine serum (FBS) were from Euroclone; lentiviral plasmids sh‐RNAs, amino acids, and blasticidine were from Sigma Aldrich; Torin 1 (cat. no. 4247) from Tocris; LysoTracker DND99 (L7528) and DQ Green BSA (D12050) were from Thermo Fisher; siRNAs were purchased as SMART pool from Dharmacon.

Di Malta C., Zampelli A., Granieri L., Vilardo C., De Cegli R., Cinque L., Nusco E., Pece S., Tosoni D., Sanguedolce F., Sorrentino N.C., Merino M.J., Nielsen D., Srinivasan R., Ball M.W., Ricketts C.J., Vocke C.D., Lang M., Karim B., Lanfrancone L., Schmidt L.S., Linehan W.M, & Ballabio A. (2023). TFEB and TFE3 drive kidney cystogenesis and tumorigenesis. EMBO Molecular Medicine, 15(5), e16877.

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