Hnrnpa0

For the preparation of immunoblotting (IB) samples, the protein of cell lysates was quantified using Easy II Protein Quantitative Kit (based on BCA; TransGen Biotech, Beijing, China). Next, the equal quantification of protein samples was electrophoresed using SDS/PAGE gel (containing 1% SDS, 1.5 m Tris pH8.8) and was transferred to nitrocellulose (NC) membrane. After blocking with 5% (w/v) skim milk at room temperature for 1 h, the membranes were incubated with primary antibodies in recommended dilution at 4 °C overnight. The next day, after washing with 1×TBST three times (each for 5 min), the membranes were incubated with HRP‐conjugated secondary antibodies (with a dilution of ~ 1 : 2000–5000) at room temperature for 1 h. After washing with 1×TBST for 3 × 5 min, the membranes were detected using Tanon™ High‐sig ECL Western Blotting Substrate Kit (Cat: 180‐501, Tanon, Shanghai, China). The protein bands were scanned and analyzed using Bio‐Rad ChemiDoc MP. Primary antibodies of HNRNPA0 (Cat: #5545), LIN28A (Cat: #8706), FLAG (Cat: #14793), COL5A1 (Cat: #86903), MMP1 (Cat: #54376), and GAPDH (Cat: #5174) were purchased from Cell Signaling Technology (Danvers, MA, USA). SPINK5 (Cat: AF8515) was purchased from R&D System (Minneapolis, MN, USA).

Recombinant human fibroblast growth factor 9 (FGF9; #273-F9-025), hepatocyte growth factor (HGF; 294-HG), transforming growth factor alpha (TGFα; 239-A) and epidermal growth factor (EGF; 236-EG) were purchased from R&D systems. Antibodies raised against phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (#9101), p44/42 MAPK (ERK1/2) (#9102), α-Tubulin (11H10) (#2125), phospho-EGF receptor (Tyr1068) (D7A5) (#3777), EGF receptor (D38B1) XP® rabbit mAb 1:1000 (#4267), GRB2 rabbit antibody (#3972), E-Cadherin (24E10) (#3195), β-Actin (13E5) (#4970), hnRNP A0 (#4046), RAS (27H5) (#3339), and GST (26H1) (#2624), (#7076) were purchased from Cell Signalling, as was an anti-rabbit IgG HRP-linked antibody (#7074). An anti-RFP rabbit antibody (ab62341) was purchased from Abcam and an anti-SOS1 mouse antibody (WH0006654M1) purchased from Sigma-Aldrich. DH5α competent and One Shot™ E. coli were purchased from Thermo Fisher Scientific. BL21 (DE3) competent E. Coli were purchased from NEB.