Cd193 bv605

Manufactured by BioLegend

CD193 BV605 is a flow cytometry reagent that is used to detect and analyze the expression of the CD193 (CCR3) surface marker on cells. CD193 is a chemokine receptor that is expressed on various immune cell types, including eosinophils, basophils, and Th2 cells. The BV605 fluorochrome is used to label the CD193 antibody, enabling the detection and quantification of CD193-positive cells by flow cytometry.

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Lab products found in correlation

Cd123 buv395, by BD (2 mentions) Cd14 pe cy5, by Thermo Fisher Scientific (2 mentions) Hla dr bv785, by BioLegend (2 mentions) Cd15 pb, by BioLegend (1 mentions) Fluorescence activated cell sorter facs lysing solution, by BD (1 mentions) Facsymphony a5 instrument, by BD (1 mentions) Trucount tube, by BD (1 mentions) Facs lysing solution, by BD (1 mentions) 6 color tbnk reagent, by BD (1 mentions)

2 protocols using cd193 bv605

Multicolor Flow Cytometry Leukocyte Profiling

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Absolute counts of peripheral blood leukocytes were determined for all study participants using BD Trucount tubes, six-color TBNK Reagent and, in addition, CD123 BUV395 (BD Biosciences), CD15 PB, CD193 BV605, and HLA-DR BV785 (Biolegend), and CD14 PE-Cy5 (eBioscience) according to the manufacturer’s instructions. Samples were fixed in 1× BD fluorescence-activated cell sorter (FACS) lysing solution (BD Biosciences) for 2 h and acquired on a BD FACSymphony A5 instrument, equipped with ultraviolet (355nm), violet (405 nm), blue (488 nm), yellow/green (561 nm), and red (637 nm) lasers. For absolute cell count calculations, the number of events for populations of interest was divided by the number of bead events and multiplied by the BD Trucount bead count.

Lourda M., Dzidic M., Hertwig L., Bergsten H., Palma Medina L.M., Sinha I., Kvedaraite E., Chen P., Muvva J.R., Gorin J.B., Cornillet M., Emgård J., Moll K., García M., Maleki K.T., Klingström J., Michaëlsson J., Flodström-Tullberg M., Brighenti S., Buggert M., Mjösberg J., Malmberg K.J., Sandberg J.K., Henter J.I., Folkesson E., Gredmark-Russ S., Sönnerborg A., Eriksson L.I., Rooyackers O., Aleman S., Strålin K., Ljunggren H.G., Björkström N.K., Svensson M., Ponzetta A., Norrby-Teglund A, & Chambers B.J. (2021). High-dimensional profiling reveals phenotypic heterogeneity and disease-specific alterations of granulocytes in COVID-19. Proceedings of the National Academy of Sciences of the United States of America, 118(40), e2109123118.

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Absolute Leukocyte Quantification via Trucount

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In order to determine the absolute leukocyte numbers, trucount staining was performed. Briefly, 50 µL EDTA blood and 20 µL of antibody mix (6-color TBNK Reagent and CD123 BUV395 from BD, CD15 PB, CD193 BV605 and HLA-DR BV785 from Biolegend, and CD14 PE-Cy5 from eBioscience) were added in BD trucount tubes and incubated for 15 min at room temperature in the dark. The samples were then fixed using 430 µL 1× BD FACS lysing solution (BD Biosciences).

Kvedaraite E., Hertwig L., Sinha I., Ponzetta A., Hed Myrberg I., Lourda M., Dzidic M., Akber M., Klingström J., Folkesson E., Muvva J.R., Chen P., Gredmark-Russ S., Brighenti S., Norrby-Teglund A., Eriksson L.I., Rooyackers O., Aleman S., Strålin K., Ljunggren H.G., Ginhoux F., Björkström N.K., Henter J.I, & Svensson M. (2021). Major alterations in the mononuclear phagocyte landscape associated with COVID-19 severity. Proceedings of the National Academy of Sciences of the United States of America, 118(6), e2018587118.

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