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Vacutainer plus plastic serum

Manufactured by BD

The BD Vacutainer Plus Plastic Serum is a laboratory blood collection tube used for the collection, transportation, and processing of human blood samples. It is designed to separate blood serum from the cellular components of the blood sample.

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2 protocols using vacutainer plus plastic serum

1

Peripheral Blood Mononuclear Cell Isolation

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About 20 mL of whole blood was collected by venipuncture in Vacutainer tubes containing EDTA (BD Biosciences, San Jose, CA, USA) at each study visit. Peripheral blood mononuclear cells were separated by Ficoll gradient centrifugation (Lympholyte; Cedarlane Labs, Hornby, ON, Canada) and washed twice in phosphate-buffered saline solution.24 Freshly isolated PBMCs were used immediately for immune phenotyping and activation staining. About 10 mL of whole blood was collected by BD Vacutainer Plus Plastic Serum. After centrifuge, serum was stored in aliquots at −80°C.
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2

PBMC Isolation and Serum Collection

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About 20 mL of whole blood was collected by venipuncture in Vacutainer tubes containing EDTA (BD Biosciences, San Jose, CA, USA) at each study visit. Peripheral blood mononuclear cells were separated by Ficoll gradient centrifugation (Lympholyte; Cedarlane Labs, Hornby, ON, Canada) and washed twice in phosphate-buffered saline solution (Ceccarelli et al. 2017b) . Freshly isolated PBMCs were used immediately for immune phenotyping and activation staining. About 10 mL of whole blood was collected by BD Vacutainer Plus Plastic Serum. After centrifuge, serum was stored in aliquots at -80 °C.
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