Dylight 649 goat anti mouse igg antibody

Manufactured by BioLegend

Sourced in United States

The DyLight 649 goat anti-mouse IgG antibody is a secondary antibody conjugated with the DyLight 649 fluorescent dye. It is designed to bind to mouse immunoglobulin G (IgG) antibodies, enabling their detection and visualization in various immunoassays and imaging applications.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Confocal imaging system, by PerkinElmer (1 mentions) Dylight 488 donkey anti rabbit igg antibody, by BioLegend (1 mentions) Anti flag tag monoclonal antibody, by Merck Group (1 mentions) Novoexpress software, by Agilent Technologies (1 mentions)

2 protocols using dylight 649 goat anti mouse igg antibody

Immunofluorescence Imaging of Fixed Cells

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The target cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized by 0.25% TritonX-100 for 10 minutes and sequentially incubated with indicated primary antibodies, secondary antibodies (DyLight 649 goat anti-mouse IgG antibody, DyLight 488 donkey anti-rabbit IgG antibody) (Biolegend, USA), and 4-6-diamidino-2- phenylindole (DAPI) (Invitrogen, USA). Images were detected by the confocal imaging system (PerkinElmer, USA). The fluorescence intensity was analyzed by ImageJ software.

Lu W., Yu T., Liu S., Li S., Li S., Liu J., Xu Y., Xing H., Tian Z., Tang K., Rao Q., Wang J, & Wang M. (2017). FHL2 interacts with iASPP and impacts the biological functions of leukemia cells. Oncotarget, 8(25), 40885-40895.

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Flag-tagged Protein Immunofluorescence Assay

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The Ba/F3 cells were transfected with Flag-tagged expression plasmids. Then the cells were permeabilized and sequentially incubated with anti-Flag-tag monoclonal antibodies (Sigma-Aldrich); secondary antibodies, including DyLight 649 goat anti-mouse IgG antibody (Biolegend); and 4-6diamidino-2-phenylindole (DAPI; Invitrogen). Images were obtained with a laser scanning confocal microscope (Leica SP2).
Flow cytometry assays were performed using ACEA NovoCyte® 3000 ow cytometer and NovoExpress software (ACEA) was used for data acquisition and analyses.

Wang Z., Liu T., Liu W., Gao X., Wan L., Qiu S., Song Y., Gu R., Tian Z., Wang M., Wang J., Mi Y, & Wei S. (2023). A novel subclonal rearrangement of the STRN3::PDGFRB gene in de novo acute myeloid leukemia with NPM1 mutation and its leukemogenic effects. Cancer gene therapy, 30(11).

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