~5 OD pellets were lysed in laemmli buffer and loaded onto 10% SDS PAGE. Protein transfer was performed onto nitrocellulose membrane using cold wet tank transfer protocol. Western blotting was performed with 1:5000 dilution of mouse monoclonal anti-DBP5 and 1:10000 dilution of mouse monoclonal anti-GAPDH (Thermo Fisher) primary antibody overnight at 4°C and 1:10000 anti-mouse DyeLight 650 secondary to detect protein.
Mouse monoclonal anti dbp5
Manufactured by Thermo Fisher Scientific
Mouse monoclonal anti-DBP5 is an antibody that specifically recognizes the DBP5 protein. DBP5 is an essential factor involved in mRNA export from the nucleus. This antibody can be used to detect and study the expression and localization of DBP5 in various biological systems.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mouse monoclonal anti dbp5
1
Western Blot Analysis of DBP5 and GAPDH
2
Western Blot Analysis of DBP5 and GAPDH
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~5 OD pellets were lysed in laemmli buffer and loaded onto 10% SDS PAGE. Protein transfer was performed onto nitrocellulose membrane using cold wet tank transfer protocol. Western blotting was performed with 1:5000 dilution of mouse monoclonal anti-DBP5 and 1:10000 dilution of mouse monoclonal anti-GAPDH (Thermo Fisher) primary antibody overnight at 4°C and 1:10000 anti-mouse DyeLight 650 secondary to detect protein.
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