Univette cuvette

Manufactured by Anton Paar

Sourced in Austria

The Univette cuvette is a disposable, single-use sample holder designed for spectroscopic measurements. It provides a standardized optical path length for accurate and reproducible absorbance or transmittance measurements in laboratory applications.

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Lab products found in correlation

Litesizer 500, by Anton Paar (2 mentions) Asap 2020, by Micromeritics (1 mentions) Kalliope software, by Anton Paar (1 mentions)

2 protocols using univette cuvette

Silica Nanoparticle Characterization

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The silica NPs
were characterized by dynamic light scattering (DLS), transmission
electron microscopy (TEM), ζ-potential measurement, and nitrogen
gas adsorption. A Litesizer 500 (Anton Paar) equipped with a 658 nm
laser was used to determine the time-correlation function of the dispersion
at a backscattering angle of 175°. For ζ-potential measurements,
Univette cuvette (Anton Paar) was used and the electrophoretic mobility
of the NPs was determined by applying a 10 V direct current across
the dispersion. The TEM imaging of the NPs was performed on a JEOL
JEM-2011. The specific surface area of the particles was determined
using nitrogen gas adsorption performed on a Micromeritics ASAP 2020.

Lee J.G., Lannigan K., Shelton W.A., Meissner J, & Bharti B. (2020). Adsorption of Myoglobin and Corona Formation on Silica Nanoparticles. Langmuir, 36(47), 14157-14165.

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Zeta Potential Characterization of EVs

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The zeta potential of three independent preparations of EVs, EV mimicking liposomes, and DOPC-Chol liposomes (n = 3) was determined with a Litesizer TM 500 (Anton Paar GmbH, Graz, Austria) using electrophoretic light scattering (ELS). After appropriate dilution in PBS samples were transferred to a Univette Cuvette (Anton Paar GmbH, Graz, Austria). The electrophoretic mobility µ was then measured after 60 seconds of equilibration time at +25 °C at an automatic voltage adjustment of 5.0 V. 100 runs were performed in series mode with three repetitions and the zeta potential ζ calculated according to the Smoluchowski equation using the Kalliope TM software (Anton Paar GmbH, Granz, Austria) provided with the instrument. The following parameters were assumed: solvent refractive index: 1.3303, solvent viscosity: η = 0.8903 mPa • s, solvent relative permittivity: εr = 78.37.

Roerig J., Schiller L., Kalwa H., Hause G., Vissiennon C., Hacker M.C., Wölk C, & Schulz-Siegmund M. (2021). A focus on critical aspects of uptake and transport of milk-derived extracellular vesicles across the Caco-2 intestinal barrier model. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 166.

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