A portion of the liver, kidney, spleen, and pancreas of mice treated with PEG-GNRs, P(Glu-co-Lys)1:5-GNRs, or P(Glu-co-Lys)1:2.5-GNRs was fixed in 10% formalin and embedded in paraffin blocks. These tissues were microtome-sectioned and stained with hematoxylin and eosin. The mounted slides were scored for adverse pathological changes on a Zeiss Axiostar light microscope.
Axiostar light microscope
Manufactured by Zeiss
The Axiostar is a light microscope designed for routine laboratory applications. It features a stable stand, coarse and fine focusing knobs, and a simple illumination system. The Axiostar is capable of providing clear, high-contrast images for a variety of sample types.
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Lab products found in correlation
2 protocols using axiostar light microscope
1
Histopathological Evaluation of Nanomaterial Toxicity
2
Microscopic Analysis of Leptolyngbya Strain
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Microscopic investigations of samples were carried out using a Zeiss Axiostar light microscope equipped with differential interference contrast at a magnification of x1000. Images of live and fresh strains were taken using a digital camera (Yashica EZF1027). Strains were drawn after observation by light microscopy in order to illustrate key strain characteristics. The Leptolyngbya sp. strain was identified based on morphological criteria, according to the taxonomic keys of [20 , 21 ]. Cell dimensions were measured using a calibrated eyepiece micrometer.
The strain was also observed by means of SEM Type JEOL JSM-5400 scanning microscope after fixation in a phosphate buffer (0.2 M, pH 7.2) containing 2.5% glutaraldehyde and following post fixation in osmium tetroxide (1%) and dehydration in an ethanol series.
The strain was also observed by means of SEM Type JEOL JSM-5400 scanning microscope after fixation in a phosphate buffer (0.2 M, pH 7.2) containing 2.5% glutaraldehyde and following post fixation in osmium tetroxide (1%) and dehydration in an ethanol series.
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