Collected samples were streaked onto: Blood agar plates, MacConkey agar plates as well as mannitol salt agar plates. All samples were incubated aerobically and anaerobically. Aerobic plates were incubated at 37 °C for 24 h whereas anaerobic plates were incubated in an anaerobic jar using anaerobic system (BD) at 37 °C for 48–72 h. Plates were examined for colony characters, cellular morphology and the purity of the culture. The suspected colonies were identified according to [7] , [13] (link), [31] .
Anaerobic system
Manufactured by BD
The Anaerobic System is a laboratory equipment designed to provide a controlled anaerobic environment for various applications. It is used to create and maintain an oxygen-free atmosphere for the cultivation and incubation of anaerobic microorganisms.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using anaerobic system
1
Bacterial Isolation and Identification Protocol
2
Endometritis in Egyptian Buffaloes
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The uterine samples were obtained from 120 Egyptian buffaloes; 60 infected with endometritis and 60 uninfected ones. Buffaloes with endometritis had signs of abnormal secretions and inflammation such as swelling, redness and hardness in uterus. The uterine samples were collected in slaughterhouse from animals after sacrificing under normal condition without any special requirement, so it is not needed to any ethical permission.
Collected samples were streaked on the Blood agar, Mac-Conkey agar and mannitol salt agar plates. All samples were incubated aerobically and anaerobically. Aerobic plates were incubated at 37°C for 24 h, whereas anaerobic plates were incubated in an anaerobic jar using anaerobic system (BD) at 37°C for 84-72 h. Plates were examined for colony characters, cellular morphology and the purity of the culture.
Collected samples were streaked on the Blood agar, Mac-Conkey agar and mannitol salt agar plates. All samples were incubated aerobically and anaerobically. Aerobic plates were incubated at 37°C for 24 h, whereas anaerobic plates were incubated in an anaerobic jar using anaerobic system (BD) at 37°C for 84-72 h. Plates were examined for colony characters, cellular morphology and the purity of the culture.
3
Bacteriological profiling of buffalo endometritis
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The uteri samples were obtained from 120 Egyptian buffaloes; 60 infected with endometritis and 60 normal ones. Buffaloes with endometritis showed abnormal secretions with signs of inflammation such as swelling, redness and hardness in the uterus.
Collected samples were streaked onto: Blood agar, Mac-Conkey agar and mannitol salt agar plates. All samples were incubated aerobically and anaerobically. Aerobic plates were incubated at 37°C for 24 h, whereas anaerobic plates were incubated in an anaerobic jar using anaerobic system (BD) at 37°C for 84-72 h. Plates were examined for colony characters, cellular morphology and the purity of the culture.
Collected samples were streaked onto: Blood agar, Mac-Conkey agar and mannitol salt agar plates. All samples were incubated aerobically and anaerobically. Aerobic plates were incubated at 37°C for 24 h, whereas anaerobic plates were incubated in an anaerobic jar using anaerobic system (BD) at 37°C for 84-72 h. Plates were examined for colony characters, cellular morphology and the purity of the culture.
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