Neuroinflammation panel 1 human kit

Manufactured by Mesoscale

Sourced in United States

The Neuroinflammation Panel-1 Human Kits are a collection of laboratory equipment designed to measure various biomarkers associated with neuroinflammation. The kits provide a standardized and efficient method for quantifying these markers in human samples.

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Lab products found in correlation

Human quantikine elisa kit, by R&D Systems (2 mentions) Quickplex sq 120, by Mesoscale (1 mentions) Meso quickplex sq 120, by Mesoscale (1 mentions)

4 protocols using neuroinflammation panel 1 human kit

Serum CRP Quantification Protocol

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Morning blood samples were used to isolate serum following standard laboratory procedures and stored at −80 °C. Serum concentrations of C-reactive protein (CRP) were analyzed in duplicate with V-PLEX Neuroinflammation Panel-1 Human Kits and a Meso Quickplex SQ120 instrument (Meso Scale Diagnostics, Rockville, MD, USA). The lowest level of quantification (LLOQ) was 0.027 mg/L, and the intra- and inter-assay coefficients of variation were 2.34% and 10.04%, respectively. CRP concentration was log-transformed, and outliers (defined as an absolute value larger than three standard deviations from the mean) were removed from analyses.

Thomas M., Savitz J., Zhang Y., Burrows K., Smith R., Figueroa-Hall L., Kuplicki R., Khalsa S.S., Taki Y., Teague T.K., Irwin M.R., Yeh F.C., Paulus M.P, & Zheng H. (2021). Elevated Systemic Inflammation Is Associated with Reduced Corticolimbic White Matter Integrity in Depression. Life, 12(1), 43.

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CRP Measurement from Morning Serum Samples

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Serum from morning blood samples was isolated following standard laboratory procedures and stored at −80 °C. CRP serum concentrations were analyzed in duplicate with the V-PLEX Neuroinflammation Panel-1 Human Kits on a Meso Quickplex SQ120 instrument (Meso Scale Diagnostics, Maryland, USA). The lowest level of quantification (LLOQ) was 27.6 pg/mL and the intra-and inter-assay coefficients of variation were 2.30% and 10.04%, respectively. CRP concentrations were log-transformed, and one participant was removed from CRP related analyses due to an absolute value larger than three standard deviations from the mean. No samples were below the LLOQ.

Zheng H., Teague T.K., Yeh F.C., Burrows K., Figueroa-Hall L.K., Aupperle R.L., Khalsa S.S., Paulus M.P, & Savitz J. (2022). C-Reactive Protein and the Kynurenic Acid to Quinolinic Acid Ratio are Independently Associated with White Matter Integrity in Major Depressive Disorder. Brain, behavior, and immunity, 105, 180-189.

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Serum Biomarkers of Metabolic Health

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Serum leptin and insulin concentrations were both analyzed using the Human Leptin, Insulin Kit (Meso Scale Diagnostics, Maryland, USA). Adiponectin was measured with the Human Quantikine ELISA kit (R&D Systems, Minneapolis, USA). The Neuroinflammation Panel 1 Human Kit (Meso Scale Diagnostics, Maryland, USA) was used to measure CRP concentrations. All analytes were tested in duplicate. The intra- and inter-assay coefficients of variation (CV) were 6.7% and 8.9% (leptin), 6.9% and 8.5% (insulin), 2.8% and 6.9% (adiponectin), 2.2% and 10.0% (CRP), respectively.

Burrows K., McNaughton B.A., Figueroa-Hall L.K., Spechler P.A., Kuplicki R., Victor T.A., Aupperle R., Khalsa S.S., Savitz J.B., Teague T.K., Paulus M.P, & Stewart J.L. (2023). Elevated serum leptin is associated with attenuated reward anticipation in major depressive disorder independent of peripheral C-reactive protein levels. Scientific Reports, 13, 11313.

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Inflammation Biomarker Measurement Protocol

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The Neuroinflammation Panel 1 Human Kit (Meso Scale Diagnostics, Maryland, USA) was used to measure 37 inflammation-related markers (full list detailed in supplemental Table S1). Interleukin 1 receptor antagonist (IL-1 ra) was measured with Human Quantikine ELISA kits (R & D Systems, Minneapolis, USA). All serum samples were tested in duplicate. IL-1α, IL-1β, IL-2 and IL-5 were not used due to a low detection rate (<80%). Inflammatory mediators IL-4, IL-10, IL-13, IL-15, IL-17A, IP-10, placental growth factor (PIGF), serum amyloid A (SAA), tyrosine protein kinase receptor Tie 2 (Tie-2), vascular endothelial growth factor C (VEGF-C) and TNF-β were excluded from the analysis due to their high inter-assay coefficients of variation (CV > 15%). We also excluded eotaxin-3 due to its high intra-assay CV (> 10%). Finally, a total of 22 analytes with an intra-assay CV <10 and an inter-assay CV < 15% were used for inflammatory mediator data analysis. Supplemental Table S2 lists CVs for each analyte used for analysis.

Burrows K., Stewart J.L., Kuplicki R., Figueroa-Hall L., Spechler P.A., Zheng H., Guinjoan S.M., Savitz J.B., Teague T.K, & Paulus M.P. (2021). Elevated peripheral inflammation is associated with attenuated striatal reward anticipation in major depressive disorder. Brain, behavior, and immunity, 93, 214-225.

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