Cannabinol

Stock methanolic solutions (1.0 mg/mL) of cannabidiol (CBD), cannabigerol (CBG) and cannabinol (CBN), as well as stock acetonitrile solutions (1.0 mg/mL) of cannabidiolic acid (CBDA) and cannabigerolic acid (CBGA), were purchased from Cerilliant Corporation (Round Rock, TX, USA).
Stock 2-propanol solutions (1.0 mg/mL) of α-pinene, β-pinene, β-myrcene, limonene, linalool, β-caryophyllene, α-humulene, caryophyllene oxide and trans-nerolidol were obtained from Agilent Technologies (Santa Clara, CA, USA).
Acetonitrile (ACN), formic acid (HCOOH), n-hexane, methanol (MeOH), 2-propanol and methyl-octanoate were purchased from Sigma Aldrich s.r.l. (Milan, Italy). Water (H₂O) was purified using a Milli-Q Plus185 system from Millipore (Milford, MA, USA).
Extra virgin olive oil was of European Pharmacopoeia grade. CBD powder (>99%) and hemp female inflorescences (Kompolti variety), listed within the EU database of admitted agricultural species, were purchased from Materia Medica Processing (Bolzano, Italy).

Certified cannabinoid reference standards (at 1 mg/mL in methanol) including cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), cannabigerol (CBG), CBD, tetrahydrocannabivarin, cannabinol (CBN), Δ⁹-THC, cannabichromene (CBC), cannabidivarin (CBDV), and Δ⁹-tetrahydrocannabinolic acid-A (THCA) were purchased from Cerilliant (Round Rock, TX). Second-source standards of CBN, CBD, and Δ⁹-THC were from Restek (Bellefonte, PA). CBD hemp oil was purchased from HempMeds (Poway, CA). Norgestrel, 4-pentylphenyl 4-methylbenzoate (PPMB), ammonium formate, formic acid, and sesame oil were from Sigma-Aldrich (St. Louis, MO). Medium-chain triglyceride (MCT) oil was from Warner Graham (Cockeysville, MD). Propylene glycol was from J.T. Baker (Central Valley, PA). Ammonium formate, formic acid, methanol, acetonitrile, and water were HPLC grade. All other reagents used were analytical grade. Bulk cannabis plant material was obtained from the National Institute on Drug Abuse (NIDA), National Institutes of Health, Bethesda, MD.

Eleven cannabinoids, namely cannabidivann (CBDV), tetrahydrocannabivarin (THCV), cannabidiol (CBD) with 99.66% purity (Lot: FE08071702), cannabigerol (CBG) with 98.98% purity (Lot: FE06241604), cannabidiolic acid (CBDA) with 98.3% purity (Lot: FE12011601), cannabigerolic acid (CBGA), cannabinol (CBN) with 99.37% purity (Lot: FE06131701), delta 9-tetrahydrocannabinol (Δ9-THC) with 97.66% purity (Lot: FE1041701), delta 8-tetrahydrocannabinol (Δ8-THC), cannabichomene (CBC) with 97.60% purity (Lot: FE10011502), and delta 9-Tetrahydrocannabinol acid (THCA-A) with 99.18% purity (Lot: FE12121601) stock solution with the concentration of 1000 µg/mL in acetonitrile or methanol (acid forms) as reference standards were purchased from Cerilliant, a Sigma Aldrich company (Kinesis Australia Pty Ltd., Redland Bay, QLD, and Novachem Pty Lt, Victoria, Australia). All other solvents (methanol, acetonitrile, and phosphoric acid) were purchased from Merck.