Antibiotic antimicotic solution

Human lung cancer cell lines (A-549, H-23, H1573, H-1373, H-1734, H-2347, H-2444, H-1650, H-522, Calu-3, H-1395, H-1435, H-1838, H-2228, H-2286, A-427) were purchased from American Type Culture Collection (ATCC). A-549 and Calu-3 cells were maintained and passaged in Dulbecco’s modified Eagle medium (DMEM) (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Gibco, Thermo Fisher Scientific) and 1% antibiotic-antimicotic solution (Gibco, Thermo Fisher Scientific) in a humidified incubator at 37°C and 5% CO₂. H-23, H1573, H-1373, H-1734, H-2347, H-2444, H-1650, H-522, H-1395, H-1838, H-2228, and A-427 cells were maintained and passaged in Roswell Park Memorial Institute–1640 medium (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS (Gibco, Thermo Fisher Scientific) and 1% antibiotic-antimicotic solution (Gibco, Thermo Fisher Scientific) in a humidified incubator at 37°C and 5% CO₂. H-1435 and H-2286 cells were maintained and passaged in DMEM/nutrient mixture F12 (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% antibiotic-antimicotic solution in a humidified incubator at 37°C and 5% CO₂. The summary of signature genetic changes of the cell lines is listed in Supplemental Table 3.