Human BCa cell lines MCF-7 and MDA-MB-231 were purchased from KeyGEN BioTECH Inc. (Nanjing, China). MCF-7 cells were cultured in RPMI-1640 media (KeyGEN BioTECH Inc.) containing 10% fetal bovine serum (FBS). MDA-MB-231 cells were cultured in L15 media (KeyGEN BioTECH Inc.) containing 10% FBS. All cells were cultured in the condition of 37 °C with 5% CO2. For subsequent assays, COL10A1-siRNA and siRNA control, synthesized in KeyGEN BioTECH Inc. (Nanjing, China), using Lipofectamine 3000 Reagent (Invitrogen) according to the manufacturer's instructions, was used to transfect incubated cells.
Rpmi 1640 media
Manufactured by Keygen Biotech
Sourced in China, United States
RPMI-1640 media is a widely used cell culture medium formulation that supports the growth and maintenance of a variety of cell types. It provides a balanced salt solution, amino acids, vitamins, and other nutrients essential for cell proliferation and survival.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by Keygen Biotech (1 mentions)
Mda mb 231, by Keygen Biotech (1 mentions)
L 15 media, by Keygen Biotech (1 mentions)
Rhodamine 6g, by Maclin Biochemical Technology (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Alkaline phosphatase, by Merck Group (1 mentions)
Trypsin, by Keygen Biotech (1 mentions)
Stearic acid, by Maclin Biochemical Technology (1 mentions)
Sodium orthovanadate, by Maclin Biochemical Technology (1 mentions)
Fetal bovine plasma, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Keygen Biotech (1 mentions)
Lb983 imaging system, by Berthold Technologies (1 mentions)
Lsm 900 confocal laser scanning microscope, by Zeiss (1 mentions)
Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
6550 qtof instrument, by Agilent Technologies (1 mentions)
4 protocols using rpmi 1640 media
1
BCa Cell Lines Transfection Assay
2
Synthesis and Characterization of Nanomaterials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zinc stearate (Zn 10–12%), sulfur powder (99.99%), Oleylamine (C18 80–90%), selenium powder (99.9%), glutathione (98%), tritonX-100 (Biochemical reagent), p-nitrophenyl phosphate disodium hexahydrate (98%) was purchased from Aladdin (ShangHai, China). Stearic acid (98%), rhodamine 6G(Analytical reagent), sodium orthovanadate (Analytical reagent) were purchased from Macklin (ShangHai, China). Alkaline Phosphatase (from bovine intestinal mucosa), Proteinase K (from tritirachium album limber) were purchased from Sigma (Saint Louis, America). Trypsin (biological reagent), pepsin (Biological reagent), RPMI1640 media were purchased from KeyGen Biotech (NanJing, China). Thrombin (from bovine plasma) and Alkaline Phosphatase assay kits were purchased from Byetime (ShangHai, China). Human serum was purchased from XinFan Biotech (ShangHai, China). Fetal bovine serum (FBS) was purchased from BIOIND (Israel). Methyl thiazolyl tetrazolium (MTT) was purchased from Biosharp (Hefei, China).
3
Culturing Diverse Lung Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Chinese Academy of Sciences Cell Bank supplied the normal human bronchial epithelial cell line (BEAS-2B, RRID: CVCL 0168), the HCC95 cells (RRID: CVCL 5137), the H2814 cells (RRID: CVCL 6898), the HCC520 cells (RRID: CVCL 1566), the LK2 cells (RRID: CVCL W132), the HCC2450 cells (RRID: CVCL 5133), BEAS-2B cells were grown in fetal bovine plasma(Gibco, Grand Island, USA)-supplemented RPMI-1640 media (Keygen Biotech, China). Other types of cancer cells (HCC95, HCC520, LK2, HCC2450, and H2887) were grown in RPMI-1640 medium (Keygen Biotech, China) with 10% fetal bovine plasma (Gibco, Grand Island, USA). Cells were maintained in a humidified 37 °C incubator with 5% CO2. The cells have been validated within the last 3 years by Guangzhou Biotech Corp. and no mycoplasma has been detected.
4
Organelle-Targeted Molecular Probes for Cell Death
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
2-Methylbenzothiazole,
4-hydroxyisophthalaldehyde, rhodamine 123, and 1,4-dioxane were purchased
from Energy Chemical (China). LysoTracker Green was purchased from
Beyotime Biotechnology Co., Ltd. (Shanghai, China). Thiazolyl blue
tetrazolium bromide (MTT) was purchased from Biosharp. Erastin and
(1S, 3R)-RSL3 (RSL3) were obtained
from Target Molecule. Dulbecco′s modified Eagle medium (DMEM)
and fetal bovine serum (FBS) were purchased from Gibco Life Technologies.
Trypsin and DiO were obtained from Beijing Solarbio Science &
Technology Co., Ltd. Newborn calf serum was purchased from Zhejiang
Tianhang Biological Technology Co., Ltd. RPMI 1640 media were purchased
from KeyGEN BioTECH Co., Ltd.
NMR spectra were recorded on an
Agilent DD2 400 MHz spectrometer. High-resolution mass spectra (HR-MS)
were obtained using an Agilent 6550 Q-TOF instrument. UV–vis
absorption spectra were performed on a TU-1901 spectrophotometer (Beijing,
China). Fluorescence spectra were recorded on a Cary Eclipse fluorescence
spectrophotometer (Varian). The MTT assay was measured by a WD-2102B
microplate reader (Liuyi, Beijing, China). Fluorescence images were
carried out on an LSM 900 confocal laser scanning microscope (CarlZeiss,
Germany) with a 63× oil-immersion objective lens and processed
with ZEN software or ImageJ software. Mice imaging was performed on
a Nightowl LB 983 imaging system (Berthold, Germany).
4-hydroxyisophthalaldehyde, rhodamine 123, and 1,4-dioxane were purchased
from Energy Chemical (China). LysoTracker Green was purchased from
Beyotime Biotechnology Co., Ltd. (Shanghai, China). Thiazolyl blue
tetrazolium bromide (MTT) was purchased from Biosharp. Erastin and
(1S, 3R)-RSL3 (RSL3) were obtained
from Target Molecule. Dulbecco′s modified Eagle medium (DMEM)
and fetal bovine serum (FBS) were purchased from Gibco Life Technologies.
Trypsin and DiO were obtained from Beijing Solarbio Science &
Technology Co., Ltd. Newborn calf serum was purchased from Zhejiang
Tianhang Biological Technology Co., Ltd. RPMI 1640 media were purchased
from KeyGEN BioTECH Co., Ltd.
NMR spectra were recorded on an
Agilent DD2 400 MHz spectrometer. High-resolution mass spectra (HR-MS)
were obtained using an Agilent 6550 Q-TOF instrument. UV–vis
absorption spectra were performed on a TU-1901 spectrophotometer (Beijing,
China). Fluorescence spectra were recorded on a Cary Eclipse fluorescence
spectrophotometer (Varian). The MTT assay was measured by a WD-2102B
microplate reader (Liuyi, Beijing, China). Fluorescence images were
carried out on an LSM 900 confocal laser scanning microscope (CarlZeiss,
Germany) with a 63× oil-immersion objective lens and processed
with ZEN software or ImageJ software. Mice imaging was performed on
a Nightowl LB 983 imaging system (Berthold, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!