Stata 12 graphics

General linear models were used to compare age-adjusted indirect measurements between groups according to hypertension, CHD, stroke, diabetes or cancer status and FLT1 genotype. Logistic models were used to compare the age-adjusted direct measurements. Cox proportional hazards models assessed the association of genotype of FLT1 with longevity using 4 genetic models; namely, major allele recessive (GG) vs other (GC, CC); heterozygote (GC) vs other (GG, CC), minor allele homozygote (CC) vs. other (GG, GC), and the additive model (number of C alleles) in which the combined effect of alleles is equal to the sum of their individual effects. The interaction of genotypes with chronic diseases such as hypertension, CHD, stroke, diabetes, and cancer was evaluated for these 4 gene models. If an interaction was significant after correction for multiple comparisons by the Bonferroni-Holm method [36 ], stratified analyses were then performed for genotype effect on mortality based on that gene model by that disease condition. Survival curves were generated using a Cox proportional hazard model adding an interaction term of FLT1 genotype with disease. All statistical analyses were performed using the Statistical Analysis System version 9.4 [37 ]. Figures were generated using STATA 12 Graphics [38 ].

General linear models were used to compare age-adjusted indirect measurements between groups, and logistic models was used to compare the age-adjusted direct measurements. Cox proportional models were used to assess the association of GHR for 3 genetic models – namely, AA vs AG/GG, AG vs AA/GG, and GG vs AA/AG – on mortality stratified by disease status, such as by diabetes, by hypertension, by CHD, and by cancer. The effects of the genotype on mortality were corrected for multiple tests using by the Bonferroni method. The significant genetic model was selected and used in the analyses. The Cox proportional hazard assumption was tested for each Cox model. The effect of interaction of disease with GHR genotype on mortality was tested in the Cox model. All statistical analyses were performed using the Statistical Analysis System version 9.4 [36 ]. Figures were generated using STATA 12 Graphics [37 ].