Lactate dehydrogenase ldh test kits

Pirarubicin and schisandrin B, purity ≥98%, were obtained from Shanghai Aladdin Reagent Co., Ltd. (Shanghai, China). Mitochondrial permeability transition pore (mPTP) inhibitor cyclosporin A (CsA) was purchased from MCE company. Brain natriuretic peptide (BNP), creatine kinase MB (CK-MB), and cardiac troponin T (cTnT) test kits were all purchased from Jiangsu enzyme label Biotechnology Co., Ltd. (Jiangsu, China). Malondialdehyde (MDA), superoxide dismutase (SOD), and lactate dehydrogenase (LDH) test kits were obtained from Nanjing Jian Cheng Biological Engineering Research Institute (Nanjing, China). The antibody of cleaved-caspase 9, pro/cleaved-caspase 3, Bcl-2/Bax, Cyt C, and COX IV were obtained from Cell Signaling Technology. All chemicals and reagents were of analytical grade.

DMEM was purchased from Thermo Fisher Biochemicals Co., Ltd. (USA). Foetal bovine serum, PLA2 enzyme, and penicillin were purchased from Gibco (USA). An AMV one-step RT-PCR kit was obtained from TaKaRa (China). Kuhuang injection extract powder was provided by Changshu Leiyunshang Pharmaceutical Co., Ltd. (China). A peristaltic pump was purchased from Master-Flex (German), and a 0.22 μm filter (Millex-GV) (German) was used. Sodium Deoxycholate (SDC) was obtained from VETEC (USA), and disposable indwelling intravenous needles (BD type Y 22 G) (USA) were used. A cell counting kit-8 (CCK-8) kit was purchased from Dojindo (USA), and reactive oxygen species (ROS) detection kits were purchased from Shanghai Biyuntian (China). Malondialdehyde (MDA) assay kits, superoxide dismutase (SOD) assay kits, glutathione (GSH) assay kits, and TBA assay kits were purchased from Nanjing Jiancheng Institute of Bioengineering (China). Aspartate transaminase (AST) test kits, alanine transaminase (ALT) test kits, lactate dehydrogenase (LDH) test kits, and alkaline phosphatase (ALP) test kits were all obtained from Nanjing Jiancheng Institute of Biological Engineering (China). CPZ was purchased from MCE (MedChemExpress) (USA), and GW9662 was obtained from Selleck (USA).