Karpas 422 cell line

The KARPAS-422 cell line was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ; Germany). Pfeiffer, Z-138, MDA-MB-468, and Toledo lines were obtained from the American Type Culture Collection (ATCC). KARPAS-422 and Pfeiffer cell lines were grown in RPMI-1640 medium (Gibco) with HEPES supplemented with 20% Fetal Bovine Serum (FBS; Sigma Aldrich), 1% Glutamax (Life Technologies), and 1% Sodium Pyruvate (Life Technologies) at 37 °C with 5% CO₂. Toledo and MDA-MB-468 cells were cultured with RPMI-1640 medium supplemented with 10% FBS. Z-138 cells were cultured with IMDM medium (Gibco) supplemented with 10% horse serum (Gibco). Every 3–4 days, when the cell cultures reached approximately 70–90% confluence, cells were split by dilution with fresh media. Cell culture density was determined using a Vi-Cell Analyzer (Beckman Coulter). For experimental assays, cells were plated to maintain subconfluence for the duration of the assay and maintained at 37 °C with 5% CO₂ for a minimum of 16 h prior to compound dosing.