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Ascorbic acid

Manufactured by Beijing Chemical Works
Sourced in China

Ascorbic acid, also known as vitamin C, is a chemical compound that is a white, crystalline solid. It is a reducing agent and antioxidant, and is commonly used in laboratory and industrial applications.

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5 protocols using ascorbic acid

1

Enzyme-Catalyzed Oxidation Assay

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All chemicals were of analytical grade and used as received without further purification. 3, 3′, 5, 5′-Tetramethylbenzidine (TMB) was purchased from BIOSHARP company, glucose oxidase (GOx, from Aspergillus niger, ≥100 U/mg), and DMPO (5, 5-dimethyl-1-pyrroline N-oxide) were purchased from Sigma-Aldrich (St. Louis, USA). Glucose, fructose, lactose, maltose, cholesterol, inositol, ascorbic acid, vitamin B1, and vitamin B9 were purchased from Beijing Chemical Reagent Company (Beijing, China). H2O2 and other regeants were obtained from Boyue Biological Reagent Co. (Harbin, China).
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2

Osteogenic Potential of Hesperidin and Icariin

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Hesperidin and icariin were purchased from Shanghai Yuan Ye Technology Co., Ltd. (batch number B20182, B21576 Shanghai, China), with a purity greater than 98%. DMEM culture medium (batch number 11965092), fetal bovine serum (batch number 12483020), and penicillin (100 U/mL) and streptomycin (100 µg/mL) (batch number 15140122) were obtained from GIBCO (New York, NY, USA). Dimethyl sulfoxide (DMSO) (batch number 472301-500ML), dexamethasone (batch number D8041), ascorbic acid (batch number A103533), β-glycerophosphate (batch number G5422-1), Calcein (batch number C302986), AAPH (batch number ajci23234), and 2,7-difluorofluorescein diacetate (DCFH-DA) (batch number D6470), prednisolone (batch number P0180), etidronate disodium (batch number B27158) were purchased from Beijing Chemical Factory Co., Ltd. (Beijing, China). The alkaline phosphatase assay kit and alkaline phosphatase chromogenic kit were obtained from Biyun Tian Biotechnology Co., Ltd. (batch number P0321S, C3206 Shanghai, China). The NO, TNF-α, and IL-6 enzyme-linked immunosorbent assay kits were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (batch number Shanghai, China).
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3

Osteogenic Potential of Hesperidin and Icariin

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Hesperidin and icariin were purchased from Shanghai Yuan Ye Technology Co., Ltd. (batch number B20182, B21576 Shanghai, China), with a purity greater than 98%. DMEM culture medium (batch number 11965092), fetal bovine serum (batch number 12483020), and penicillin (100 U/mL) and streptomycin (100 µg/mL) (batch number 15140122) were obtained from GIBCO (New York, NY, USA). Dimethyl sulfoxide (DMSO) (batch number 472301-500ML), dexamethasone (batch number D8041), ascorbic acid (batch number A103533), β-glycerophosphate (batch number G5422-1), Calcein (batch number C302986), AAPH (batch number ajci23234), and 2,7-difluorofluorescein diacetate (DCFH-DA) (batch number D6470), prednisolone (batch number P0180), etidronate disodium (batch number B27158) were purchased from Beijing Chemical Factory Co., Ltd. (Beijing, China). The alkaline phosphatase assay kit and alkaline phosphatase chromogenic kit were obtained from Biyun Tian Biotechnology Co., Ltd. (batch number P0321S, C3206 Shanghai, China). The NO, TNF-α, and IL-6 enzyme-linked immunosorbent assay kits were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (batch number Shanghai, China).
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4

Versatile Porphyrin-based Nanocomposite Synthesis

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All chemicals were used as received without further purification. Ferric chloride hexahydrate (FeCl3.6H2O), 5, 10, 15, 20-tetra (4-(imidazol-1-yl)phenyl) porphyrindine (TIPP), polyvinylpyrrolidone (PVP, Mw~4000) and tetraethyl orthosilicate (TEOS) were purchased from Aladdin Company. Ethanol (CH3CH2OH, 99.7%), N, N-dimethylformamide (DMF, AR), α,α‘-Dibromo-p-xylene, sodium sulfate anhydrous (Na2SO4), sodium hydroxide (NaOH), hydrogen chloride (HCl, 37%) were purchased from Sinopharm Chemical Reagent Co., Ltd. Chloroauric acid (HAuCl4·4H2O) was purchased from Sigma-Aldrich. Dopamine HCl was purchased from Beijing HWRK Chem Co., Ltd. 3,3’,5,5’-tetramethylbenzidine (TMB) and PBS were gotten from Sangon Biotech (Shanghai) Co., Ltd. Glucose, fructose, sucrose, L-cysteine, ascorbic acid, dopamine, uric acid, and maltose were purchased from Beijing Chemical Reagent Company. Deionized (DI) water from Milli-Q System (18.2 MΩ·cm, Millipore, Billerica, MA) was used in all experiments.
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5

Graphene Oxide Composite Synthesis

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Concentrated sulfuric acid (H 2 SO 4 , 98%), nitric acid (HNO 3 , 70%), sodium nitrate (NaNO 3 ), potassium permanganate (KMnO 4 ), hydrogen peroxide (H 2 O 2 , 38%), hydrochloric acid (HCl, 38%), ethanol, and ascorbic acid were purchased from Beijing Chemical Factory (China). Pristine graphite was supplied by Huadong Graphite Factory (China). Epoxy resin (JY-257) with an epoxy value of 0.53-0.55 mol/100g was obtained from Jiafa Chemical Co. (China). Methyl hexahydrophthalic anhydride as the curing agent and tris-(dimethyl aminomethyl) phenol as the curing accelerator were purchased from Alfa Aesar (China).
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